Compound pesticide containing triflumizole and ningnanmycin
A technology of Ningnanmycin and fluconazole, applied in the field of pesticides
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Embodiment 1
[0045] Embodiment 1 The preparation of the composition containing Ningnanmycin
[0046] Inoculate the frozen Streptomyces noursii var. Xichang (purchased from Heilongjiang Biopesticide Engineering Technology Research Center) on a freshly prepared solid medium, at 28 ° C, 80% relative humidity, and under the condition of sterile air circulation Cultured for 16 days for rejuvenation, and the resulting strain spores were picked. Then produce mycelium, that is, to inoculate the spores of the strain on the freshly prepared solid medium, and culture it aerobically at 28°C, 80% relative humidity, and under the condition of sterile air circulation for 14 days, and the mycelium can be clearly seen produce. Carry out proliferation culture then, promptly use the first freshly prepared liquid medium to wash mycelia from the surface of the solid medium and enter a 1L Erlenmeyer flask, add the first freshly prepared liquid medium to supplement to a medium volume of 250ml, Incubate for 14 ...
Embodiment 2
[0050] The formula of embodiment 2 compound pesticides
[0051] 1. 25% fluconazole + 4% Ningnanmycin wettable powder (WP): take fluconazole 25g; Ningnanmycin 4g prepared in Example 1; sodium lauryl sulfate (wetting agent) 2g ; Sodium lignosulfonate (dispersant) 4g; Sodium methylene dinaphthalene sulfonate (NNO, dispersant) 5g; Kaolin (filler) made up to 100g, crushed into wettable powder.
[0052] 2. 25% fluconazole + 4% Ningnanmycin suspension concentrate (SC): take flucloxanil 25g; Ningnanmycin 4g prepared in Example 1; sodium lignosulfonate (dispersant) 3g; methylene Sodium dinaphthalenesulfonate (NNO, dispersant) 2g; ethylene glycol (antifreeze) 4g; xanthan gum (thickener) 0.2g; silicone oil (defoamer) 0.3g; deionized water to make up to 100g.
[0053] 3. 15% fluconazole+6% Ningnanmycin microemulsion (ME): get flucloxanil 15g; Ningnanmycin 6g prepared in Example 1; xylene (solvent) 10g; dodecylbenzene Calcium sulfonate (emulsifier) 5g; phenylethylphenol polyoxyethylene e...
Embodiment 3
[0056] The control effect of embodiment 3 compound pesticides
[0057] We dilute the 25% flufenazole+4% Ningnanmycin wettable powder (WP1) prepared according to Example 2 with an equivalent amount of commercially available 30% fluconazole wettable powder (purchased from Nippon Soda Co., Ltd. ) (WP2) and 25% fluconazole + 4% Ningnanmycin wettable powder (WP3) prepared with the original drug; it refers to the corresponding formula of Example 2, but with Ningnanmycin Seoul Biochemical Technology Development Co., Ltd.) instead) to do field experiments to show its control effect on various crops.
[0058] (1) Field efficacy test of rice stripe leaf blight
[0059] In the high-incidence area of Henan Province, 2 mu of rice test field was divided into equal parts, and each area was tested with WP1, WP2, WP3 and blank control (CP). After 7 days, apply the same amount of pesticide again, and observe the effect of controlling rice stripe leaf blight on the 7th day after the last app...
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