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Artificial surface-active substance mixed liquid as well as preparation and application thereof

A technology of active substances and artificial surfaces, applied in the direction of drug combinations, peptide/protein ingredients, medical preparations of non-active ingredients, etc., can solve problems such as weak effects

Inactive Publication Date: 2011-05-11
陕西麦科奥特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hydrophilic protein SP-A also has similar physiological functions, but its effect is weaker than that of SP-B and SP-C

Method used

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  • Artificial surface-active substance mixed liquid as well as preparation and application thereof
  • Artificial surface-active substance mixed liquid as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 The mixed solution of pulmonary surfactant and its preparation

[0025] Recipe: DPPC 23g

[0026] POPG 8g

[0027] Oleic acid 4g

[0028] SP-B 1g

[0029] Phosphate buffer to 100g

[0030] Preparation: Make a solvent with the ratio of chloroform:methanol=2:1 (v / v); dissolve DPPC and POPG in the solvent according to the above ratio, mix well, quickly evaporate to remove the organic solvent, and freeze-dry at low temperature to obtain powdered phospholipid; Add 1% SP-B and oleic acid to phosphate buffer solution, add mixed freeze-dried DPPC and POPG components, stir in a warm bath at 40°C until completely dissolved, and make the final concentration reach SP-B1-2%, DPPC 20-30 %, POPG5-15%, for distribution.

Embodiment 2

[0031] Embodiment 2 The mixed solution of pulmonary surfactant and its preparation

[0032] Recipe: DPPC 23g

[0033] POPG 8g

[0034] Oleic acid 4g

[0035] SP-B 0.5g

[0036] SP-C 0.5g

[0037] Phosphate buffer to 100g

[0038] Preparation: Make a solvent with the ratio of chloroform:methanol=2:1 (v / v); dissolve DPPC, POPG, and oleic acid into the solvent according to the above ratio, mix well, quickly evaporate to remove the organic solvent, and freeze-dry at low temperature to obtain powder Phospholipids; add 0.5% SP-B and 0.5% SP-C to phosphate buffer, add mixed freeze-dried DPPC and POPG components, stir in a 40°C warm bath until completely dissolved, and make the final concentration reach SP-B0. 5-1%, SP-C0.5-1%, DPPC 20-30%, POPG5-15%, for separate equipment.

Embodiment 3

[0039] Embodiment 3 The mixed solution of pulmonary surfactant and its preparation

[0040]Recipe: DPPC 23g

[0041] POPG 8g

[0042] Oleic acid 5g

[0043] KL 4 1g

[0044] Tris Buffer Add to 100g

[0045] Preparation: Make a solvent with the ratio of chloroform:methanol=2:1 (v / v); dissolve DPPC, POPG, and oleic acid into the solvent according to the above ratio, mix well, quickly evaporate to remove the organic solvent, and freeze-dry at low temperature to obtain powder Phospholipids; Phosphate buffered saline with 1% KL 4 , add the mixed freeze-dried DPPC and POPG components, stir in a 40°C warm bath until completely dissolved, and make the final concentration reach KL 4 1-2%, DPPC 20-30%, POPG5-15%, for separate equipment.

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Abstract

The invention relates to an artificial surface-active substance mixed liquid as well as preparation and application thereof. The artificial surface-active substance mixed liquid disclosed by the invention is prepared from surfactant protein B (SP-B) / surfactant protein C (SP-C) or natural polypeptide with surface activity, lipids, surface active agents and a buffer solution. The artificial surface-active substance mixed liquid is suitable for respiratory distress syndrome for new born children or adults and other diseases relevant to deficiency of surface substances or abnormal functions.

Description

Technical field: [0001] The invention relates to the field of pharmaceutical preparations, and relates to the proportion of a mixed liquid containing pulmonary surfactant, its preparation method and its application in medicine. Background technique: [0002] Pulmonary surfactant (PS) is mainly a lipoprotein complex synthesized and secreted by alveolar type II cells, and is an important substance for maintaining normal respiratory function. PS is a complex phospholipid, containing 85% to 90% phospholipid, 8% to 10% protein, 5% neutral fat and a small amount of inorganic salt. In phospholipids, phosphatidylcholine (Phosphatidyleholine, PC) accounts for 60% to 50%, phosphatidylglycerol (Phosphatidylglycerol, PG) accounts for 5% to 10%, also includes phosphatidylinositol (phosphatidylinositol, PI), sphingomyelin (Sphingomyelin, SPH), phosphatidylserine (Phosphatidylsersne, PSe), etc. PC is the main component of phospholipids, in which double-saturated phosphatidylcholine, that...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K47/24A61K47/28A61K47/12A61P11/00
Inventor 郝心宇王梅王大璋
Owner 陕西麦科奥特生物科技有限公司
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