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Application of formononetin in preparing of medicament for restricting angiogenesis

A technology of formononetin and angiogenesis, which is applied in the direction of drug combination, cardiovascular system diseases, anti-tumor drugs, etc., to achieve the effect of inhibiting proliferation

Inactive Publication Date: 2011-05-11
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no report about the application of formononetin in inhibiting angiogenesis

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  • Application of formononetin in preparing of medicament for restricting angiogenesis
  • Application of formononetin in preparing of medicament for restricting angiogenesis
  • Application of formononetin in preparing of medicament for restricting angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Formononetin inhibits the proliferation of human umbilical vein endothelial cells (HUVEC)

[0033] The proliferation of HUVEC was detected by CCK-8 method. CCK-8 reagent contains WST–8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene) -2H-tetrazolium monosodium salt), which is reduced by the dehydrogenase in the mitochondria of the cell under the action of the electron carrier 1-Methoxy PMS (1-methoxy-5-methylphenazine dimethyl sulfate) to have Highly water-soluble yellow formazan (Formazan) product. The amount of formazan produced is directly proportional to the number of viable cells. The light absorption value was measured at a wavelength of 450 nm by an enzyme-linked immunosorbent detector, which can indirectly reflect the number of living cells. The effect of drugs on the proliferation of HUVCE can be evaluated by this method.

[0034] Methods: HUVEC cells were seeded in 96-well plates and cultured. After the cell growt...

Embodiment 2

[0038] Example 2 Formononetin inhibits the migration of human umbilical vein endothelial cells (HUVEC)

[0039] Transwell method was used to detect HUVEC migration. Transwell is an experimental technology. The main material of this technology is the Transwell chamber (chamber). The bottom layer of the chamber has a layer of permeable membrane (polycarbonate membrane (polycarbonate membrane) is commonly used, and this membrane has apertures. Micropores with a size of 0.1-12.0 μm. In the experiment, the cells are placed on the small chamber. Under the action of the chemokines or growth factors contained in the small chamber, the cells pass through the polycarbonate membrane, thereby performing co-culture, cell chemotaxis, cell Migration, cell invasion and many other studies.

[0040] Method: Add 600 μL of VEGF containing 20 ng / ml to the lower chamber 165 HUVEC culture medium. Add HUVEC containing different concentrations of formononetin in the upper chamber (cell density is...

Embodiment 3

[0042] Example 3 Experiment of formononetin inhibiting tubule formation in HUVEC

[0043] The tubule formation of HUVEC was detected by Matrigel gel. Artificial basement membrane Matrigel is a basement membrane component extracted from mouse EHS sarcoma. It can spontaneously form a gel-like artificial basement membrane at 37 °C, and has the biological function of a natural basement membrane. Human umbilical vein endothelial cells can adhere to form tubes on Matrigel glue, which can be used to study the effect of drugs on the tube formation of endothelial cells.

[0044] Method: 50 μl of Matrigel gel solution was added to a pre-cooled 96-well culture plate, and then placed in a 37 °C incubator for 1 h to solidify the gel. HUVEC containing different concentrations of formononetin (cell density 1×10 5 / ml) were added to the 96-well plate covered with Matrigel, placed in CO 2 Incubate for 10 h in the cell culture incubator. The group without formononetin was used as control....

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Abstract

The invention relates to application of formononetin in preparing of a medicament for restricting angiogenesis. The invention has the advantages that: the new application of the formononetin is provided, the fact that the formononetin can effectively restrict the propagation and the migration of endothelial cells in veins and canaliculization is found first, and firstly found in the invention, the formononetin has an anti-angiogenesis activity and can be used as an angiogenesis depressor. The invention firstly finds that the formononetin has the anti-angiogenesis action and can be applied to the preparation of the angiogenesis medicament as an angiogenesis depressor so as to treat angiogenesis-dependent and angiogenesis-associated diseases, such as tumors, arthritis, psoriasis, eye diseases, atherosclerosis, and the like.

Description

technical field [0001] The invention relates to the application of a Chinese herbal medicine monomer, in particular, the application of a Chinese herbal medicine monomer formononetin in the preparation of an angiogenesis-inhibiting drug. Background technique [0002] Angiogenesis is the process by which new blood vessels develop from existing capillaries. Angiogenesis is a complex process involving a variety of cells and molecules, mainly including the following processes: vascular basement membrane degradation in the activation phase; activation, proliferation, and migration of vascular endothelial cells; and reconstruction of new blood vessels and vascular networks. [0003] Blood vessels are an important way to transport oxygen, nutrients and metabolites, and it is difficult for tissues or organs without vascular support to survive. [0004] Neovascularization occurs under both physiological and pathological conditions. Physiological angiogenesis mainly occurs during em...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00A61P35/02A61P17/06A61P19/02A61P9/10A61P27/02
Inventor 赵梅陈红专方超陆琴白帆王超於得红
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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