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Antiphase high efficiency liquid chromatography method for segregating polymyxin E methyl sodium sulfnate and analog thereof

A high-performance liquid chromatography and high-performance liquid analysis technology, which can be used in the field of high-performance liquid chromatography for separation and analysis of polymyxin E sodium methanesulfonate and its isomers or analogs, and in the field of chromatographic separation and analysis, and can solve toxic and side effects Large, low toxicity, application has not been widely promoted and other problems

Inactive Publication Date: 2011-04-27
杨更亮
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  • Description
  • Claims
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Problems solved by technology

However, due to its high toxicity and side effects, its application has not been widely promoted.
The polymyxin E sodium methanesulfonate synthesized from it as a raw material is less toxic than it, and the antibacterial effect is not reduced
At present, there is no analytical method for this type of drug in China. Therefore, the analysis and research of this type of drug has become a work of great significance at present.

Method used

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  • Antiphase high efficiency liquid chromatography method for segregating polymyxin E methyl sodium sulfnate and analog thereof
  • Antiphase high efficiency liquid chromatography method for segregating polymyxin E methyl sodium sulfnate and analog thereof
  • Antiphase high efficiency liquid chromatography method for segregating polymyxin E methyl sodium sulfnate and analog thereof

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Embodiment Construction

[0055] The above specific examples can further illustrate the present invention and its application, but are not intended to limit the scope of protection of the claims of the present invention.

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Abstract

The invention discloses a method for segregating and analyzing polymyxin E methyl sodium sulfnate and isomer or analog thereof successfully by utilizing alkyl bonded silica packed column. The method provided by the invention is characterized in that the condition of the liquid chromatography is that the alkyl bonded silica packed column is a stationary phase, and a mobile phase is a mixed solvent composed of an organic solvent and aqueous acid, wherein the C18 alkyl bonded silica gel packed column of Optimapak corporation is taken as the stationary phase, and when acetonitrile and 0.05% of trifluoroacetic acid water solution gradient are eluted based on the volume ratio of (20:80)-(50:50), the good segregation effect is achieved.

Description

technical field [0001] The invention relates to a chromatographic separation and analysis method, in particular to a high performance liquid chromatography (HPLC) method for separation and analysis of polymyxin E sodium methanesulfonate and its isomers or analogues, and belongs to the field of drug analysis. Background technique [0002] Many serious infections are caused by highly resistant Pseudomonas aeruginosa, and colistin drugs have a good inhibitory effect on the pathogenic bacteria of such infections. Since polymyxin sulfate compounds were extracted from different species of Bacillus polymyxa in 1947, they have been used in in vitro antibacterial experiments. However, due to its high toxicity and side effects, its application has not been widely promoted. The polymyxin E sodium methanesulfonate synthesized from it as a raw material is less toxic than it, and the antibacterial effect is not reduced. At present, there is no analytical method for this type of drug in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/50G01N30/60G01N30/36
Inventor 杨更亮白立改马正月杨静闫宏远
Owner 杨更亮
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