Immunochromatographic test strip for full quantitative detection of C-reactive protein and preparation method thereof

Abstract

The invention discloses an immunochromatographic test strip for full quantitative detection of C-reactive protein and a preparation method thereof. The test strip is composed of a sample pad, a mark pad, a coating film and absorbent paper which are successively overlapped and adhered on a baseplate, wherein the mark pad is coated with C-reactive protein (CRP) monoclonal antibody labelled by fluorescent latex particles and rabbit IgG labelled by fluorescent latex particles; and the coating film is composed of a detection region and a quality control region, and the detection region is coated with another CRP monoclonal antibody which is at different epitope with the CRP monoclonal antibody labelled by fluorescent latex particles. The C-reactive protein immunochromatographic test strip can perform sensitive quantitative detection on the full CRP in 10 seconds, can diagnose diseases and identify infection more rapidly and accurately, and can detect infectious illness state and determine the curative effect of antibiotics; and the full CRP has two detection results, namely hs-CRP and conventional CRP, comprehensive linear range and good detection sensitivity and less required sample amount, and is very convenient to operate.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to an immunochromatographic test strip for quantitatively detecting C-reactive protein throughout the whole process and a preparation method thereof. Background technique [0002] C-reactive protein (C-reactive protein, CRP), named after Tillet and Francis found in the serum of patients with acute lobar pneumonia in 1930 that it can react with pneumococcal C polysaccharide in the presence of calcium ions, is an important acute human Phase response protein, the acute phase concentration can be increased by thousands of times, and the half-life of CRP in the circulation is 19 hours. [0003] C-reactive protein can be combined with the capsular C polysaccharide of Streptococcus pneumoniae. It is an acute phase protein synthesized by liver cells when the body is stimulated by microbial invasion or tissue damage. When inflammatory diseases or tissue ulceration or necrosis occur, it will be...

AI Technical Summary

Problems solved by technology

The routine clinical method for measuring C-reactive protein is the immunoturbidimetric method, and the measurement range is generally 3-200 mg / L, but its sensitivity is relatively low, and it is impossible to accurately measure the content of C-reactive protein below 3 mg / L. Risk indicators of vascular diseases, predicting the risk of cardiovascular and cerebrovascular diseases

The detection range of clinical high-sensitivity C-reactive protein is relatively narrow, and it is impossible to accurately measure the content of CRP at high concentrations, so it cannot be used for the diagnosis of infectious diseases or the observation of curative effect

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