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Melamine test paper, preparation method thereof, and method for detecting melamine

A technology of melamine and test paper, which is applied in the field of analysis and detection, can solve the problems of limited shelf life and high cost

Inactive Publication Date: 2011-04-20
LIAONING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0013] The detection reaction principles of the two quick test cards and the two test papers all involve enzyme reaction or immune reaction, which are expensive and have a limited shelf life.

Method used

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  • Melamine test paper, preparation method thereof, and method for detecting melamine

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Embodiment Construction

[0030] Melamine spiked control experiment detection and analysis, the instrument used is the UV762 ultraviolet / visible spectrometer produced by Shanghai Precision Instrument Co., Ltd.

[0031] 1. Preparation method of standard milk supernatant containing melamine:

[0032] Accurately weigh 8 copies of 3.2000g commercially available milk powder (Wandashan brand zinc-added milk powder, Heilongjiang Wandashan Dairy Co., Ltd., product standard number: Q / WDS 001, production date: September 11, 2010), in In a small beaker, add boiling water to dissolve it, add 0, 0.0100, 0.0200, 0.0300, 0.0400, 0.0500, 0.0600, 0.0800g melamine respectively, and add 0.4000g EDTA, heat to a slight boil for 5min, stand still, settle, filter, and use Wash the insoluble matter with hot distilled water, collect the filtrate in a 100mL volumetric flask, and add water to volume. Obtain No. 1 → No. 8 standard milk supernatant containing melamine, the concentration of melamine is respectively 0, 0.1000, 0.20...

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Abstract

The invention provides melamine test paper and a preparation method thereof, and a method for detecting the melamine. The melamine test paper is prepared by the following steps of: preparing alizarin red, methylene blue and andlauryl sodium sulphate into an aqueous solution, wherein the concentration of each substance in the aqueous solution is: 0.0167 mol / L of the alizarin red, 2.10*10<-5> mol / L of the methylene blue and 0.015 mol / L of the andlauryl sodium sulphate; immersing filter paper into the aqueous solution, taking out and drying; and shearing into strips to obtain the melamine test paper. When the melamine test paper is in use, the method for detecting the melamine comprises the steps of: adding EDTA (Ethylene Diamine Tetraacetic Acid) solid powder into the to-be-detected liquid cream which is prepared from milk or by mixing milk powder; evenly agitating and heating and micro boiling for 5 min; statically placing and depositing; immersing the test paper into the liquid cream liquid supernatant; and wetting and then extracting out. The invention has the advantages of simple using method, and high detecting speed.

Description

technical field [0001] The invention belongs to the analysis and detection technology and is used for the detection of food safety. Background technique [0002] Melamine (melamine) is referred to as triamine for short, also known as melamine, melamine, and cyanuronamide. It is a triazine nitrogen-containing heterocyclic organic compound, and its nitrogen content is as high as 66%. Melamine is a pure white monoclinic crystal, tasteless, because the traditional protein content determination method in the food industry cannot detect the type of nitrogen, because melamine has a high nitrogen content, it is often used as a food additive by unscrupulous businessmen to pretend to be protein in food, so Melamine is also known as "protein essence". In March 2007, many cats, dogs and other pet poisoning deaths occurred in the United States. The main reason was that melamine was added to pet food. Melamine can decompose at high temperature to produce cyanide. After eating, animals ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 赫春香李翱楠刘洋
Owner LIAONING NORMAL UNIVERSITY
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