Method for recovering glucomannan and protein in konjac flying powder by enzyme process
A technology of konjac flying powder and glucomannan, which is applied in animal feed, animal husbandry, application, etc., can solve problems such as technical difficulties in protein and konjac glucomannan recovery, improve resource utilization efficiency, reduce pollution, and facilitate The effect of the operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0030] Ingredients: Weigh 5g of konjac powder, add it into a beaker with 200 mL of water, and stir it fully with an electric stirrer to obtain an aqueous solution of konjac powder;
[0031] Liquefaction: Heat the aqueous solution of konjac powder in a water bath to 45°C, adjust the pH to 4.0, add fungal α-amylase, the amount of fungal α-amylase is 10 U / g konjac powder, and liquefy for 2 hours. Until the starch is fully degraded (judged by iodine-potassium iodide reagent: add iodine-potassium iodide reagent to the solution, if no blue color appears in the solution, it indicates that the starch has been fully degraded), the solution viscosity decreases significantly, and the reducing sugar content increases;
[0032] Saccharification: adjust the temperature of the liquefied solution to 40°C, adjust the pH to 3.0, add amyloglucosidase, the amount of amyloglucosidase is 10 U / g konjac powder, stir evenly, and continue to react for 24 hours;
[0033] Centrifugation: adjust the pH of...
Embodiment 2
[0038] Ingredients: Weigh 5g of konjac flying powder, add it to a beaker with 1000 mL of water, and use an electric stirrer to fully stir it evenly to obtain an aqueous solution of konjac flying powder;
[0039] Liquefaction: Heat the aqueous solution of konjac powder to 65°C in a water bath, adjust the pH to 6.6, add fungal α-amylase, the amount of fungal α-amylase is 500 U / g konjac powder, react for 2 hours, until The starch is fully degraded (judged by iodine-potassium iodide solution), the viscosity of the solution is significantly reduced, and the content of reducing sugar is increased;
[0040] Saccharification: adjust the temperature of the liquefied solution to 60°C, adjust the pH to 5.5, add amyloglucosidase, the amount of amyloglucosidase is 500 U / g konjac fly powder, stir evenly, and continue to react for 3 hours;
[0041] Centrifugation: adjust the pH of the saccharified solution to 5, set the centrifugation parameters as: centrifugation rate 5000 rpm / min, centrifu...
Embodiment 3
[0046] Ingredients: Weigh 5g of konjac flying powder, add it to a beaker with 400 mL of water, and use an electric stirrer to fully stir it evenly to obtain an aqueous solution of konjac flying powder;
[0047] Liquefaction: Heat the aqueous solution of konjac powder in a water bath to 50°C, adjust the pH to 5.0, add medium-temperature α-amylase, the amount of medium-temperature α-amylase is 10 U / g konjac powder, react for 2 hours, until The starch is fully degraded (judged by iodine-potassium iodide solution), the viscosity of the solution is significantly reduced, and the content of reducing sugar is increased;
[0048] Saccharification: adjust the temperature of the liquefied solution to 40°C, adjust the pH to 3.0, add amyloglucosidase, the amount of amyloglucosidase is 10 U / g konjac powder, stir evenly, and continue to react for 24 hours;
[0049] Centrifugation: adjust the pH of the saccharified solution to 3.5, set the centrifugation parameters as follows: centrifugation...
PUM
Property | Measurement | Unit |
---|---|---|
viscosity | aaaaa | aaaaa |
recovery rate | aaaaa | aaaaa |
recovery rate | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com