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Method for removing phenol in waste water by enzyme catalysis

A waste water and phenol technology, applied in chemical instruments and methods, water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of environmental secondary pollution, mucous membrane irritation, skin sensitization, etc., and achieve reduction Effects of operating costs, mitigation of passivation, reduction of reaction time

Inactive Publication Date: 2011-04-20
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these chemical surfactants often have toxic side effects, such as irritation to the mucous membrane, sensitization to the skin, carcinogenicity, teratogenicity, hemolysis, etc.
They will cause secondary pollution of the environment, which is limited in practical promotion

Method used

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  • Method for removing phenol in waste water by enzyme catalysis
  • Method for removing phenol in waste water by enzyme catalysis
  • Method for removing phenol in waste water by enzyme catalysis

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0021] In this example, two schemes of adding and not adding dirhamnolipid were implemented respectively, and the embodiment without adding dirhamnolipid was used as a comparative example to illustrate the removal effect of phenol in wastewater.

[0022] According to the method of enzyme-catalyzed removal of phenol in waste water of the present invention (when adding dirhamnolipid), the method specifically comprises the following steps:

[0023] (1) Select wastewater with a phenol concentration of 100 mg / l, then add dirhamnolipid to a concentration of 210 mg / l, and then add laccase to a concentration of 1.15 U / ml;

[0024] (2) Adjust the pH value of the wastewater to 6 with NaOH or HCl, and control the temperature of the wastewater at 25°C;

[0025] (3) Vigorously stir the above wastewater for 15 minutes, then let it stand still, and select four different time points of 2h, 6h, 10h and 24h for the standstill (reaction) time to investigate;

[0026] (4) Select a filter membran...

Embodiment 2

[0030] In this example, two schemes of adding and not adding dirhamnolipid were also implemented respectively, and the embodiment without adding dirhamnolipid was used as a comparative example to illustrate the removal effect of phenol in wastewater.

[0031] According to the method of enzyme-catalyzed removal of phenol in waste water of the present invention (when adding dirhamnolipid), the method specifically comprises the following steps:

[0032] (1) Select wastewater with a phenol concentration of 400 mg / l, then add dirhamnolipid to a concentration of 210 mg / l, and then add laccase to a concentration of 1.15 U / ml;

[0033] (2) Adjust the pH value of the wastewater to 6 with NaOH or HCl, and control the temperature of the wastewater at 25°C;

[0034] (3) Vigorously stir the above wastewater for 15 minutes, then let it stand still, and select four different time points of 2h, 6h, 10h and 24h for the standstill (reaction) time to investigate;

[0035] (4) Select a filter me...

Embodiment 3

[0039] In this example, two schemes of adding and not adding dirhamnolipid were also implemented respectively, and the embodiment without adding dirhamnolipid was used as a comparative example to illustrate the removal effect of phenol in wastewater.

[0040] According to the method of enzyme-catalyzed removal of phenol in waste water of the present invention (when adding dirhamnolipid), the method specifically comprises the following steps:

[0041] (1) Select wastewater with a phenol concentration of 50 mg / l, then add dirhamnolipid to a concentration of 210 mg / l, and then add laccase to a concentration of 1.15 U / ml;

[0042] (2) Adjust the pH value of the wastewater to 3, 4, 5, 6, 7, 8 and 9 with NaOH or HCl, respectively, and control the temperature of the wastewater at 25°C;

[0043] (3) Vigorously stir the wastewater at different pH values ​​above for 15 minutes, and then let it stand for 6 hours;

[0044] (4) Select a filter membrane of 0.45 μm, and pass the above-menti...

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Abstract

The invention discloses a method for removing phenol in waste water by enzyme catalysis. Biosurfactant di-rhamnolipid is mainly utilized to promote laccase catalysis, namely the di-rhamnolipid and the laccase are added in the waste water containing the phenol, the pH value of the waste water is adjusted to 4-7, and the temperature of liquid is controlled to be 25-50 DEG C; and then the mixed waste water is drastically stirred for more than 15 minutes, the liquid passes through a filter membrane with below 0.45mum after standing reaction is carried out, so as to remove the phenol in the waste water. In the processing method in the invention, the phenol removal effect is good, the efficiency is high, the operation is simple and the cost is low.

Description

technical field [0001] The invention relates to the field of wastewater treatment, in particular to an enzyme-catalyzed method for removing phenol in wastewater. Background technique [0002] Phenolic pollutants widely exist in production activities such as industry and agriculture. Phenolics are important pollutants because they are toxic, carcinogenic, and teratogenic at very low concentrations. These pollutants, if discharged into the environment, can accumulate in environments such as groundwater, surface water and soil. Therefore, the environmental treatment of phenolic pollutants has attracted more and more attention. In recent years, the method of removing phenolic pollutants by enzymatic catalysis has attracted more and more attention. These enzymes mainly include laccase, peroxidase and tyrosinase. However, some unfavorable factors in the reaction process lead to the inactivation of the enzyme, thereby reducing the efficiency of removing phenolic pollutants, inc...

Claims

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Application Information

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IPC IPC(8): C02F1/58
Inventor 刘智峰曾光明袁兴中钟华李建兵周梅芳马小玲黄璐
Owner HUNAN UNIV
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