Foam carbon biological support, and preparation method and application thereof

Abstract

The invention discloses a foam carbon biological support, and a preparation method and an application thereof. The foam carbon biological support is prepared by carbonizing a precursor formed by the foaming and solidification of phenolic resin under ordinary pressure. The method comprises the following steps: when preparation, firstly preparing materials; and then arranging into water bath for foaming and solidifying; drying and solidifying to acquire the precursor; carbonizing the precursor to obtain the foam carbon biological support. When the foam carbon biological support is used for exo-cell culture, the foam carbon biological support planted with cells is arranged in a static or dynamic circulating culture system for culturing according to the demands in different cell types. The foam carbon biological support has good aperture ratio and biocompatibility and high mechanical strength, and can be widely used for exo-cell culture.

Description

technical field [0001] The invention relates to a tissue engineering biological material and its preparation method and application, in particular to a biological scaffold and its preparation method and application. Background technique [0002] Large-scale animal cell culture is the basis of tissue engineering and genetic engineering, and a large number of biomedical experiments and in vitro pharmacological experiments are also inseparable from in vitro cell culture. The current international two-dimensional planar cell culture method in vitro obtains cells as planar monolayer cells. The life state of the cells is completely different from the three-dimensional structure in the real human body. The mutual spatial position, signal transduction and proliferation between cells Regulatory and internal environment are very different, life activities will be different from normal cell state and may lead to degeneration of cell tissue. Therefore, in order to achieve a breakthroug...

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Problems solved by technology

[0003] Three-dimensional scaffold materials that have been extensively studied at present, such as polylactide (PGA), poly-L-lactic acid (PLLA), poly-D-lactic acid (PDLA), bioactive ceramics, etc., have high specific surface area, three-dimensional interconnected pore network and biological phase Capacitive, mostly used for repairing small-scale tissue defects in the body, but due to the degradability, absorbability and low compressive strength of these scaffold materials, it is difficult to really be used as a scaffold material for high-density and long-term culture of cells in vitro

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