Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Calf in vitro embryo culture solution

A technology of embryo culture medium and culture medium, which is applied in the field of culture medium, can solve the problems of reducing the blastocyst development rate, and achieve the effect of high blastocyst development rate

Inactive Publication Date: 2011-02-02
北京奶牛中心
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the addition of cysteamine should be appropriate, otherwise it will reduce the rate of blastocyst development

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Calf in vitro embryo culture solution
  • Calf in vitro embryo culture solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Calf Hormone Superovulation and Egg Collection

[0031] On the day of calf superovulation, CIDR for sheep (for sheep, place of origin New Zealand) was implanted transvaginally with a suppository, and FSH (FSH, trade name Folltronpin-V, purchased from Canada Bioniche AnimalHealth Company), eggs were collected on the 7th day, and the thrombus was withdrawn at the same time.

[0032] The number of usable oocytes obtained in each calf super-rowing was 48 pieces.

Embodiment 2

[0033] Example 2 In vitro maturation of calf oocytes

[0034] 2 hours before the in vitro maturation operation, put an appropriate amount of maturation culture medium into each well of a 6-well conical culture plate (average 10 μl of maturation culture medium per oocyte), cover with mineral oil, and put it in a 38.5°C incubator for pre-equilibration . Among them, the composition of the mature medium is: TCM199+10mMHEPES+5%FBS+0.5μg / ml FSH+5μg / ml LH+1μg / ml E 2 + 27.5 μg / ml sodium pyruvate + 100 IU / ml penicillin + 100 μM cysteamine.

[0035] Put the identified and usable oocytes into the preheated maturation medium, wash them gently 4 times, transfer them into the maturation medium of a 6-well conical culture plate that has been equilibrated in the incubator for 2 hours, and place them in a constant temperature incubator Cultivate for 22h-24h. Culture conditions are 38.5°C, 5% CO 2 , saturated humidity. figure 1 Shown is the oocyte obtained after hormone superovulation of t...

Embodiment 3

[0036] Example 3 In vitro fertilization of calf oocytes

[0037] First, mature oocytes were mixed with fertilization fluid (114.0mM NaCl+4.02mM KCl+2.25mM CaCl 2 .2H 2 O+0.52mM MgCl 2 6H 2 O+0.83mM NaH 2 PO 4 h 2 O+37.0mM NaHCO 3 + 13.9mM glucose + 0.5mM sodium pyruvate + 3mg / mlBSA + 10μg / ml phenol red + 100IU / ml penicillin + 10μg / ml heparin), wash 2-3 times, and then put into the pre-balanced 2h fertilized fluid Medium (15 oocytes are placed in every 50ul fertilized fluid).

[0038]Take a thin tube (0.25ml) of frozen semen (the semen comes from the frozen semen of bulls from the Beijing Dairy Cow Center), put it in a water bath at 37°C for about 10 seconds, take it out until there are bubbles floating up, dry it with a facial tissue, and disinfect it with an alcohol cotton ball slim tube. The balanced centrifuge tube (containing semen washing solution: 114.0mM NaCl+4.02mM KCl+2.25mM CaCl 2 .2H 2 O+0.52mM MgCl 2 6H 2 O+0.83mM NaH 2 PO 4 h 2 O+37.0mM NaHCO 3 +1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a calf in vitro embryo culture solution containing cysteamine. The early-stage embryo in vitro development culture solution is used to culture the calf in vitro embryo and can obtain higher blastaea developmental rate.

Description

technical field [0001] The invention relates to a culture solution, in particular to a calf in vitro embryo culture solution. Background technique [0002] Like most mammals, bovine follicles occur in the fetal period, and the presence of antral follicles can be detected in the ovary of the fetus in the late pregnancy and a few days after birth, and the number reaches about 50 at the age of 2 months, which is obviously high. in adult cows. Therefore, making full use of the physiological characteristics that the production efficiency of calf oocytes is several times higher than that of adult cows, and exerting the maximum genetic potential of fine-bred calves has become one of the research hotspots in the field of dairy cow breeding technology. [0003] Using calves as donors can not only provide a large number of oocyte resources for in vitro production of embryos, but also meet the needs of scientific research and production such as oocyte freezing, embryo segmentation, cl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/073
Inventor 朱玉林吕小青薛建华宣柏华李艳华周高举吴胜权赵鹏梁鸿斌麻柱
Owner 北京奶牛中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com