Method for inducing spawning of parent Solen grandis and collecting fertilized eggs
A technology for fertilized eggs and large bamboo clams, which is applied to the field of artificial induction and breeding of large bamboo clams, can solve the problems that the two shells cannot be completely closed, the large bamboo clams are thin and fragile, and the mortality rate of the parent clams is high. The effect of high rate and high survival rate of seedlings
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Embodiment 1
[0027] Collection of shellfish: Begin to collect shellfish one after another from the first ten days of April. The shellfish is obtained by manual excavation. The surface of the shell should be smooth, the feet and appearance should be free of mechanical damage, healthy and vigorous, and the shell length should be 8-11cm;
[0028] Cultivation of broiler shellfish to promote maturation: broiler shellfish are placed in the oxytocin tank, which is required to be long and the bottom of the pool is inclined at an angle of 15° to the horizontal. The bottom of the pool is covered with 15cm thick coarse sand (0.5mm2 , change the water once a day, and the water change volume is 80%; the bait density is kept at 5-10×10 4 cell / ml, continuous aerated culture, the survival rate of temporary culture can reach more than 95%;
[0029] Oxygenation: Oxygenation can be initiated when the plumpness of the broodstock reaches over 85%, and the gonads are dissected and the sperm and eggs are self-flo...
Embodiment 2
[0032] The broodfish collected in late April were placed in the spawning pool for temporary raising, and the area of the spawning pool was 12m 2 , the depth of the pool is 1.3 meters. After nearly 50 days of temporary cultivation, labor was induced on the morning of June 17, 2009. A small amount of eggs were laid at 8:20. When the fertilized eggs reached 15 / ml at 11:00, the siphon principle was used. Quickly move the fertilized egg into the cultivation pool 109#. The area of each breeding pond is 23.8m 2 , 1.3 meters deep in the pond, the bottom of the cultivation pond is 60cm deeper than the bottom of the oxytocin pond. The fertilized eggs in the spawning pool can all be moved into the breeding pool. Immediately add sand and filter seawater to 109# quickly until the water level is 80cm, and the hatching density is 6 / ml.
[0033] After the ovum in the spawning pool was removed, the same method was used to add algae liquid (pH value 9.3) to the spawning pool, followed by...
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