Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing organic acid by fermenting abandoned thallus with monosodium glutamate

A technology of waste bacteria and organic acids, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of consuming cultivation time, material energy, consumption, etc., to reduce production costs, reduce pollution, and control carbon The effect of emissions

Inactive Publication Date: 2012-09-05
NANJING TECH UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the bacterial strains currently used for fermentative production of succinic acid are mainly bacterial strains or genetically engineered bacteria screened from characteristic anaerobic environments (rumen, stomach, etc.). And increase, in order to maintain a relatively stable high yield of succinic acid, it is necessary to make the fermentation cells reach a high concentration of cell density, this process will consume a lot of cultivation time and material energy consumption in actual production, so how to reduce the cell density The cost of enrichment has become a key problem to be solved in the industrialization of organic acid fermentation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Collect the waste cells of Corynebacterium glutamicum ATCC 10128 used in monosodium glutamate industry to ferment L-glutamic acid, and finally obtain a biomass of 10g-dry cell×L -1 . (OD 600nm =0.25g-dry cell×L -1 ).

[0017] Fully suspend the sludge with an equal volume of anaerobic medium at the end of aerobic conditions, transfer it to an anaerobic fermentation tank, and then add CO 2 The gas flows into the anaerobic fermentation medium of the fermenter at an aeration rate of 0.2v / vm, ​​the stirring speed is 200rpm, the temperature is controlled at 30°C, and the anaerobic fermentation is carried out for 24 hours.

[0018] Anaerobic fermentation medium: glucose 40g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O 6mg / L, MnSO 4 ·H 2 O 6mg / L, biotin 0.2mg / L, vitamin B1 200μg / L, NaHCO 3 42g / L. Initial pH 7.0.

[0019] As a result of fermentation, the main conversion products are lactic acid, acetic acid and succinic a...

Embodiment 2

[0021] Collect the waste cells of Corynebacterium glutamicum ATCC 10128 used in monosodium glutamate industry to ferment L-glutamic acid, and finally obtain a biomass of 10g-dry cell×L -1 . (OD 600nm =0.25g-dry cell×L -1 ).

[0022] Fully suspend the sludge with an equal volume of anaerobic medium at the end of aerobic conditions, transfer it to an anaerobic fermentation tank, and then add CO 2 The gas flows into the anaerobic fermentation medium of the fermenter at an aeration rate of 0.2v / vm, ​​the stirring speed is 200rpm, the temperature is controlled at 30°C, and the anaerobic fermentation is carried out for 32 hours.

[0023] Anaerobic fermentation medium: glucose 80g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O 6mg / L, MnSO 4 ·H 2O 6mg / L, biotin 0.2mg / L, vitamin B1 200μg / L, NaHCO 3 42g / L. Initial pH 7.0.

[0024] As a result of fermentation, the main conversion products are lactic acid, acetic acid and succinic ac...

Embodiment 3

[0026] Collect the waste cells of Corynebacterium glutamicum ATCC 10129 used in MSG industry to ferment L-glutamic acid, and finally obtain a biomass of 10g-dry cell×L -1 . (OD 600nm =0.25g-dry cell×L -1 ).

[0027] Suspend the treated sludge with an equal volume of anaerobic medium at the end of aerobic conditions, transfer it to an anaerobic fermentation tank, and then add CO 2 The gas flows into the anaerobic fermentation medium in the fermenter at an aeration rate of 0.2v / vm, ​​the stirring speed is 200rpm, the temperature is controlled at 30°C, and the anaerobic fermentation is carried out for 28 hours.

[0028] Anaerobic fermentation medium: glucose 40g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O 6mg / L, MnSO 4 ·H 2 O 6mg / L, biotin 0.2mg / L, vitamin B1 200μg / L, NaHCO 3 42g / L. Initial pH 7.0.

[0029] As a result of fermentation, the main conversion products are lactic acid, acetic acid and succinic acid, the yields...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing organic acid by fermenting abandoned thallus with monosodium glutamate. During monosodium glutamate production, corynebacterium glutamicum is fermented under the aerobic condition to produce L-glutamic acid; the abandoned thallus after the aerobic process ends can be recovered to be converted into the anaerobic condition to produce organic acid. Bacterium mud is suspended by an anaerobic medium; after being inoculated, CO2 gas is fed to the fermentation medium in a fermentation tank; at the temperature of 30 DEG C, stirring and cultivating for 24-48 hours; and maintaining the pH value of the fermentation liquid to 5.5-7.0. The result of the prepared product is analyzed, and corynebacterium glutamicum can obviously accumulate succinic acid, lactic acid and acetic acid under the anaerobic condition.

Description

technical field [0001] The invention relates to a method for fermenting organic acids in the field of biochemical industry, in particular to a method for producing organic acids by using monosodium glutamate to ferment waste cells Corynebacterium glutamicum. Background technique [0002] Corynebacterium glutamicum is a very important production strain for the fermentation of L-glutamic acid in the MSG industry. The fermentation of Corynebacterium glutamicum to produce glutamic acid is an aerobic process that produces a large amount of biomass cells. In the current process, a large amount of biomass (discarded bacteria) produced after fermentation is used to produce bacterial protein feed for animal husbandry, or to extract low-value products such as nucleotides, and the resource utilization rate is low. Generally, 8-10 kg of nucleosides (150 yuan / kg) can be recovered from one ton of waste cells fermented by monosodium glutamate, and the rest can be used as protein feed (2000...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/46C12P7/54C12P7/56C12R1/15
CPCY02P20/141
Inventor 严明郝宁许晟刘兆星蔡萍李艳欧阳平凯
Owner NANJING TECH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com