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Mass spectrum detection method of serum polypeptide

A technology for mass spectrometry detection and serum peptides, which is applied in the field of mass spectrometry detection of serum peptides, can solve the problem of limited number of cases and achieve the effect of improving the discovery rate

Inactive Publication Date: 2011-01-05
ZHEJIANG CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the number of cases used for research is relatively limited, so there will be certain limitations

Method used

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  • Mass spectrum detection method of serum polypeptide
  • Mass spectrum detection method of serum polypeptide
  • Mass spectrum detection method of serum polypeptide

Examples

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Comparison scheme
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Embodiment 1

[0030] The mass spectrometry detection method of serum polypeptide of the present invention, its specific steps are as follows:

[0031] First, divide the selected magnetic beads (WCX magnetic beads from Dynal Company) into polymerase chain reaction tubes (PCR tubes) according to 32ul / tube (the magnetic beads should be mixed evenly before dispensing), and put the PCR tubes into Store in the refrigerator at 4°C until use;

[0032] Take a portion of the PCR tube packed with magnetic beads and place it on the magnetic processor, and remove the liquid in the PCR tube after 1-2 minutes;

[0033] Take the PCR tube out of the magnetic processor, add 100 μL of 50mmol / L sodium acetate buffer (pH 4.5) into the PCR tube, mix well and let it stand at room temperature for 3-5 minutes, then place the PCR tube on the magnetic processor , remove the liquid in the PCR tube after 1-2 minutes, repeat the above operation once;

[0034] Take out the PCR tube from the magnetic processor, add 100 ...

Embodiment 2

[0042] Serum samples from 42 patients with gastric cancer (including 12 cases of stage I, 6 cases of stage II, 18 cases of stage III, and 6 cases of stage IV, half male and half female) and 42 healthy people with matched sex and age were used as serum samples. Using the same method as in Example 1 to detect one by one. The measured serum peptide spectrum was standardized by the original protein fingerprint, and then analyzed by Biomarker Wizard software. A total of 75 protein peaks were detected in the molecular weight range of 2,000 to 30,000, of which 13 peaks were different from those in the gastric cancer group and the healthy control group. There was a very significant difference in group comparison (P<0.001). The abundance of different peaks is shown in Table 1. Table 1 shows the difference peaks of serum protein between 42 patients with gastric cancer and 42 healthy people.

[0043] Table 1

[0044]

[0045] Statistically, all P<0.001.

[0046] In order to further ev...

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Abstract

The invention discloses a mass spectrum detection method of serum polypeptide. The method comprises the following steps: the magnetic bead supported matrix method is adopted to capture the gastric cancer peptidome in serum, a magnetic separator is used to separate magnetic beads and the sample without centrifuging the sample; and then the mass spectrum method is adopted to analyse the polypeptide spectrum. The method of the invention can be used to detect the serum samples of normal persons and patients with gastric cancer, and the detection result can be used as an important detection basis for the diagnosis of early gastric cancer. The detection method of the invention is accurate, convenient and fast.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a mass spectrometry detection method for serum polypeptides. Background technique [0002] Gastric cancer is one of the common malignant tumors of the digestive tract. In 2006, there were nearly 930,000 new gastric cancer cases and 700,000 deaths worldwide. In my country, the mortality rate of gastric cancer in men (40.8 / 100,000) and women (18.6 / 100,000) is 4.2-7.9 times and 3.8-8.0 times that of developed countries in Europe and America, respectively. It can be seen that the mortality rate of gastric cancer in my country is relatively high, and the situation is grim. Surgery is the only curative method for gastric cancer. Because it is often diagnosed at an advanced stage, the chance of complete surgical resection is lost. Therefore, early diagnosis and early treatment are of great significance to improve the long-term survival rate of gastric cancer patients. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N1/28G01N33/68
Inventor 余传定郑智国许沈华徐琦朱赤红
Owner ZHEJIANG CANCER HOSPITAL
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