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Nitrogen-fixing bacillus megaterium strain DL7 and application thereof

A technology of Bacillus megaterium and strain, which is applied to nitrogen-fixing Bacillus megaterium strain DL7 and its application fields, can solve the problems of reduced soil fertility, reduced productivity, poor water retention capacity of forest land, etc.

Inactive Publication Date: 2010-12-15
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to overharvesting, full reclamation and unreasonable fertilization can also lead to the decline of eucalyptus plantation
(2) Moisture problem
[0004] Eucalyptus is a non-legume plant that does not have the ability to fix nitrogen and self-fertilize. At present, the fast growth and high yield of eucalyptus plantation completely depends on the application of N, P, and K compound fertilizers, which leads to the lack of trace elements in the soil, the reduction of soil fertility, and the decline in productivity; a large amount of chemical fertilizers, Water problems caused by soil compaction, soil structure damage, and poor woodland water retention capacity

Method used

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  • Nitrogen-fixing bacillus megaterium strain DL7 and application thereof
  • Nitrogen-fixing bacillus megaterium strain DL7 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The culture method of nitrogen-fixing bacillus megaterium strain (Bacillus megaterium) DL7 of the present invention, comprises the steps:

[0024] 1. Isolation and purification of nitrogen-fixing Bacillus megaterium strain DL7

[0025] The fine roots with nodules were collected from the lateral roots of Podocarpus brevifolia, rinsed with tap water, sterilized with 75% ethanol for 1 minute, and then sterilized with 0.1% mercuric chloride for 5 to 6 minutes. Grind the root nodules and fine roots in a sterilized mortar, add a small amount of sterile water, grind well, cover and let stand, and clarify for a while. Take the supernatant and inoculate it on the YMA medium that has been added with Congo red by streaking method, culture at 28°C for 2-3 days, select the colonies that do not absorb Congo red, repeat the streaking and screening culture, and then select round, milky white, Mucilaginous, well-margined colonies. Microscopically, there are flagella, motile, and no sp...

Embodiment 2

[0031] Effects of Inoculation of Nitrogen-fixing Bacillus megaterium Strain DL7 on Eucalyptus Seedlings by Soaking Roots

[0032] This experiment was carried out in the nursery of Guangxi State-run Gaofeng Forest Farm located in the suburbs of Nanning.

[0033] The seedlings used in the test came from the Guanglin Eucalyptus grandis No. 9 (GLGU9) clone tissue culture bottle seedlings produced by the Guangxi State-run Gaofeng Forest Farm Tissue Culture Factory. After the tissue culture bottle seedlings are removed from the bottle, the culture medium is cleaned with tap water first, then sterilized with thiophanate-methyl, and the disinfectant on the surface of the seedlings is filtered for later use.

[0034] Root soaking method inoculation: before transplanting the above-treated seedlings into the nutrient cup, soak the roots of the seedlings with nitrogen-fixing Bacillus megaterium strain DL7 for 20 to 30 minutes, and then plant them in the nutrient cup. For yellow heart soi...

Embodiment 3

[0040] Effects of inoculating nitrogen-fixing Bacillus megaterium strain DL7 on Eucalyptus seedlings by spraying method.

[0041] This experiment was carried out in the nursery of Nanning Vegetable Research Institute in the suburb of Nanning.

[0042] The seedlings used in the test came from the Guanglin Eucalyptus grandis No. 9 (GLGU9) clone tissue culture bottle seedlings produced by the Guangxi State-run Gaofeng Forest Farm Tissue Culture Factory. After the tissue culture bottle seedlings are removed from the bottle, the culture medium is cleaned with tap water first, then sterilized with thiophanate-methyl, and the disinfectant on the surface of the seedlings is filtered for later use.

[0043] Spray inoculation method: After the seedlings are transplanted into the nutrition cup, use a watering can to evenly spray the nitrogen-fixing Bacillus megaterium strain DL7 on the eucalyptus seedlings planted in the nutrition cup, inoculate a total of 20,000 seedlings, and do not wa...

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Abstract

The invention relates to a nitrogen-fixing bacillus megaterium strain DL7 and application thereof. The strain is preserved in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms in June 7, 2010 and has a preserving number as CGMCC NO.3914. The strain is a new nitrogen-fixing strain obtained by using the conventional microorganism separation and purification method for Chinese podocarpus root nodules. In the strain, the nitrogenase activity is 1.5632nmol.g<-1>.h<-1>; after an eucalyptus seedling is planted, the seedling height increases 40.78-56.22 percent, the ground diameter increases 39.41-46.43 percent, the average biomass increases 72.11-158.76 percent, the chlorophyll content increases 12.97-20.98 percent, and the nitrate reductase activity increases 33.82-35.22 percent. The strain can be applied to a biological bacterial fertilizer for promoting eucalyptus seedling growth.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nitrogen-fixing Bacillus megaterium strain (Bacillus megaterium) DL7 and its application. Background technique [0002] At present, there are about 50 million hm in the world 2 of tropical plantations, and an annual increase of 3 million hm 2 left and right (Binkley et al., 2004). Among them, eucalyptus (Eucalyptus sp.) accounts for about 40% of the total tropical plantation area, and accounts for more than 50% of the timber output, and is playing an increasingly important role. The area of ​​eucalyptus plantations in China has reached 1.7 million hectares 2 Eucalyptus has become one of the three major afforestation tree species in my country, and its afforestation area is second only to India and Brazil, ranking third in the world. Eucalyptus is fast-growing, high-yielding, high-quality, and the average annual growth of the optimal hybrid family can reach 69.8m 3 / hm 2 , the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/11
Inventor 吕成群黄宝灵
Owner GUANGXI UNIV
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