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Extraction method of cellulase for improving hydrolysis of lignocellulose

A technology of lignocellulose and cellulase, applied in the field of biochemistry, can solve the problems of cost increase, separation cost, recycling, comprehensive utilization, etc.

Inactive Publication Date: 2012-12-12
GEOTHINK ECOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the addition of polymer or non-ionic surfactant also increases the cost of the additive itself, the cost of separating the additive in the enzymatic hydrolysis solution, and the impact of the residual additive in the hydrolyzed glucose solution on the next step of sugar fermentation and the recovery of cellulase Utilization, comprehensive utilization of lignin after hydrolysis of lignocellulose, etc.

Method used

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  • Extraction method of cellulase for improving hydrolysis of lignocellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Adding 5 g / 100 ml of cellulose Avicel PH 101 and 2 ml / 100 ml cellulase concentration to obtain glucose concentration of 3 g / 100 ml after reacting for 72 hours under conventional conditions.

Embodiment 2

[0030] The addition amount of cellulose Avicel PH 101 is 5g / 100ml, the addition amount of lignin is 1g / 100ml, and the concentration of cellulase is 2ml / 100ml, and the glucose concentration is 2.3g / 100ml after reacting for 72 hours according to conventional conditions.

Embodiment 3

[0032] The concentration of glucose was 1.3g / 100ml after reacting for 72 hours under conventional conditions under the condition that the addition amount of dilute acid pretreated lignocellulose was 5g / 100ml and the concentration of cellulase was 2ml / 100ml.

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PUM

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Abstract

The invention provides an extraction method of cellulose for improving the hydrolysis of lignocellulose, relating to the technical filed of biochemistry. In the process of enzyming lignocellulose, polymer or nonionic surfactant capable of being separated are added to selectively extract the lignocellulose so as to improve the hydrolysis efficiency of the lignocellulose by the cellulose, thus further separating the lignocellulose and the cellulose so as to realize recovery and reutilization of added polymer or nonionic surfactant and obtain byproduct lignocellulose; and meanwhile, hydrolyzed glucose solution can be directly used for sugar fermentation process in the next step. The method realizes the deposition of lignocellulose and obtains byproduct lignocellulose, copolymer L64 is recovered and recycled, and lignocellulose is recovered to hydrolyze lignocellulose for the next cycle.

Description

technical field [0001] The invention relates to an extraction method in the field of biochemical technology, in particular to an extraction method for improving cellulase hydrolysis of lignocellulose. Background technique [0002] The basic process of using biomass to produce bioenergy (ethanol, butanol, etc.) is pretreatment of biomass, enzymatic hydrolysis of lignocellulose after pretreatment, fermentation of glucose in enzymatic hydrolyzate, and separation of products in fermentation broth Four steps such as purification and purification, among which pretreatment of biomass and enzymatic hydrolysis of lignocellulose are the key to restricting its industrialization cost. Biomass pretreatment technology directly affects the content of lignin in the enzymatic hydrolysis of lignocellulose in the next step. The existing technology to remove lignin in lignocellulose has the disadvantages of large resource consumption, serious environmental pollution, and high cost. Generally, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P7/06
CPCY02E50/17Y02E50/10
Inventor 王志龙
Owner GEOTHINK ECOTECH SHANGHAI
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