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Pine ectomycorrhizal fungi capsule bactericide and application thereof

A technology for ectomycorrhizal fungi and pine trees, applied in the application, biocide, plant growth regulator and other directions, can solve the problems of difficult storage, inconvenient transportation, long period of the production process of inoculants, etc. Characteristics, the effect of extending the shelf life

Active Publication Date: 2010-11-24
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a pine ectomycorrhizal fungus capsule inoculum with good application effect and more convenient transportation and storage, so as to overcome the long period of production process of the current inoculum, difficult storage and inconvenient transportation, etc. defect

Method used

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  • Pine ectomycorrhizal fungi capsule bactericide and application thereof
  • Pine ectomycorrhizal fungi capsule bactericide and application thereof
  • Pine ectomycorrhizal fungi capsule bactericide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) For every 1L of water, add 40g of sodium alginate and 15g of activated carbon to the water, and mix well;

[0031] (2) A kind of ectomycorrhizal fungus of pine tree——Boletus edulis (Boletus edulis) is carried out liquid culture for a period of time under more suitable conditions, the mycelial ball is filtered out, washed 3 times with sterile water, and then Pour into a homogenizer to crush the mycelium balls, and mix the crushed mycelium with the mixture obtained in step (1) at a volume ratio of 2:5 to form a mycorrhizal gelatin;

[0032] (3) The mycorrhizal bacteria glue solution prepared in step (2) is sucked with a syringe and then added dropwise to 60g / L of CaCl 2 In the solution, spherical colloidal particles are formed, and the particle size of the prepared capsule inoculum is controlled at about 3mm. 2 After being solidified in the solution for 24 hours, it was washed 3 times with sterile water to obtain the delicious boletus capsule inoculum.

Embodiment 2

[0034] (1) For every 1L of water, add 20g of sodium alginate and 10g of activated carbon to the water, and mix well;

[0035] (2) A kind of ectomycorrhizal fungus of pine tree——Boletus edulis (Boletus edulis) is carried out liquid culture for a period of time under more suitable conditions, the mycelial ball is filtered out, washed 3 times with sterile water, and then Pour into a homogenizer to crush the mycelium balls, and mix the crushed mycelium with the mixture obtained in step (1) at a volume ratio of 3:5 to form a mycorrhizal gelatin;

[0036] (3) The mycorrhizal bacteria glue solution prepared in step (2) is sucked with a syringe and then added dropwise to 50g / L of CaCl 2 In the solution, spherical colloidal particles are formed, and the particle size of the prepared capsule inoculum is controlled at about 3mm. 2 After being solidified in the solution for 28 hours, it was washed 3 times with sterile water to obtain the delicious boletus capsule inoculum.

Embodiment 3

[0038] (1) For every 1L of water, add 80g of sodium alginate and 30g of activated carbon to the water, and mix well;

[0039] (2) A kind of ectomycorrhizal fungus of pine tree——Boletus edulis (Boletus edulis) is carried out liquid culture for a period of time under more suitable conditions, the mycelial ball is filtered out, washed 3 times with sterile water, and then Pour into a homogenizer to crush the mycelium balls, and mix the crushed mycelium with the mixture obtained in step (1) at a volume ratio of 2:5 to form a mycorrhizal gelatin;

[0040] (3) The mycorrhizal mycorrhizal glue solution prepared in step (2) is sucked with a syringe and then added dropwise to 80g / L of CaCl 2In the solution, spherical colloidal particles are formed, and the particle size of the prepared capsule inoculum is controlled at about 3mm. 2 After being solidified in the solution for 30 hours, it was washed 3 times with sterile water to obtain the delicious boletus capsule inoculum.

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Abstract

The invention discloses pine ectomycorrhizal fungi capsule bactericide, which is prepared by the following preparation processes of: crushing pine ectomycorrhizal fungi mycelium in a homogenizer, and fully mixing the crushed mycelium and a mixture of sodium alginate solution and active carbon uniformly in a volume ratio of 2-3: 5 to form mycorrhizal fungi colloidal solution; and adding the mycorrhizal fungi colloidal solution into CaCl2 solution to form spherical colloidal particles, curing the colloidal particles for 24 to 30 hours, and then washing the colloidal particles by using sterile water to obtain the bactericide. The invention also discloses application of the pine ectomycorrhizal fungi capsule bactericide in promoting pine growth. The mycorrhizal fungi capsule bactericide has the advantages of high vitality, simple preparation process, convenient material source, easy storage and easy industrialized production and has good application prospect, and the storage life of hypha is prolonged during storage.

Description

technical field [0001] The invention relates to a pine ectomycorrhizal agent and its application. Specifically, it provides a formulation that is more convenient for transportation and storage for excellent ectomycorrhizal fungi with growth-promoting and stress-resistant effects, and is suitable for production and application in pine seedling cultivation and afforestation. Background technique [0002] Pine is one of the main afforestation tree species in my country. As an excellent tree species that is resistant to barrenness and strong stress resistance, pine trees can grow under very harsh environmental conditions, especially in sandy soil and saline-alkali soil and other soil conditions that provide fertilizer and poor fertilizer retention. Therefore, it plays a pivotal role in my country's forestry resources and economic development. [0003] These advantages of pine are all related to the mycorrhizal root system. Pinus species are considered to be typical ectomycorrh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/28A01P21/00
Inventor 吴小芹周婷曾丽琼叶建仁任嘉红
Owner NANJING FORESTRY UNIV
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