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Overcoming method of character inheritance instability of cabbage type rape yellow seed

A genetically unstable, cabbage-type rape technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of unstable yellow seed rate and low yellow seed degree of cabbage-type rapeseed, and achieve feed utilization. High value, low melanin content, effect of seed coat

Inactive Publication Date: 2010-10-27
SOUTHWEST UNIVERSITY
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to solve the problem of unstable yellow seed rate and low yellow seed degree of Brassica napus, and provide a method for overcoming the genetic instability of yellow seed traits of Brassica napus, so as to breed yellow seed rate and yellow seed. The parent material with high seed quality, coordinated genetic background and yellow seed main effect gene provides abundant breeding materials for high-quality and high-yield yellow seed rape breeding and heterosis utilization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Brassica-type yellow-seed material × cabbage-type black-seed material, hybrid offspring bred into a cabbage-type brown-yellow seed material, using this material as the female parent, crossed with 20 cabbage-type black-seeded materials, observing the grain color performance of the F2 generation, and selecting the middle grain color Better single plant selfing, after 3 generations of directional selection, the lines with better grain color and more stable genetics in the offspring of different combinations were crossed with each other, and 2 rounds of recrossing were used to aggregate the yellow and brown seed materials from 8 different sources. Seed micro-effect multiple genes, and finally bred a cabbage-type yellow-seeded double-low strain. After 5 consecutive generations of observations, the yellow seed rate of this strain was 100% (no black and brown seeds in the offspring), and the yellow seed degree reached 90% (close to the cabbage-type yellow seed). the bright yell...

example 2

[0026] Cabbage-type yellow-seeded material×Brassica-type black-seeded material, the hybrid offspring bred into a cabbage-type brown-yellow-seeded material, using this material as the female parent, crossed with 10 cabbage-type black-seeded materials, observing the grain color performance of the F2 generation, and selecting the middle grain color Better single plant selfing, after 3 generations of directional selection, the lines with better grain color and more stable genetics among the progeny of different combinations are crossed and recrossed with each other, and yellow seeds from different sources of brown and yellow seed materials are aggregated. Finally, a cabbage-type yellow-seeded double-low strain was bred. After 5 consecutive generations of observations on this strain, the yellow seed rate was 100% (the offspring had no dark brown seeds), and the yellow seed degree reached 90% (close to the bright yellow color of cabbage-type yellow seeds). ).

example 3

[0028] The Canadian cabbage-type yellow-seeded material Sban was used as the male parent to cross with 8 Brassica napus lines, and the offspring underwent 8 generations of self-crossing and directional selection to obtain a batch of brown-yellow and genetically stable cabbage-type yellow-seeded materials. After 2 rounds of recrossing, yellow-seed micro-efficiency polygenes from 4 brown-yellow-seed materials were aggregated, and the offspring underwent 5 generations of self-crossing and directional selection, and finally a cabbage-type yellow-seed double-low line was bred. The line has been observed for 4 consecutive generations, and the yellow seed rate is 100%, the yellow seed degree reaches 90%, and the agronomic yield traits are good.

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Abstract

The invention relates to an overcoming method of character inheritance instability of a cabbage type rape yellow seed. In the method, cabbage type rape black seed materials having yellow seed minor genes with greater action is identified through the importation of yellow seed major genes, and then the beneficial minor polygenes are pyramided to one material through pyramiding breeding, and thus, the yellow seed material contains the yellow seed major genes in Chinese cabbage (leaf mustard) type rape and the yellow seed minor polygenes of different sites in multiple cabbage type rapes, which makes the synthesis of seedcoat pigments in the materials hindered and environmental influence action become small, thereby preparing the cabbage type rape yellow seed material with stable inheritance. The penetrance of the yellow seed can reach 100 percent and the yellow seed degree (the yellow ratio of the surface of the seed) can reach more than 80 percent.

Description

technical field [0001] The invention relates to the field of crop genetic breeding and agricultural application biotechnology, in particular to a method for overcoming the genetic instability of yellow seed traits of Brassica napus. Background technique [0002] Brassica oleracea has a series of outstanding advantages: low melanin content, thin seed skin, high oil content; clear and transparent oil, which not only maintains the original fragrance of rapeseed oil, but also has the appearance of salad oil; high protein content of cake; The content of cellulose and polyphenols is low, and the value of feed utilization is high. Moreover, yellow-seed hybrid rapeseed also has three major advantages for industrialization: (1) yellow hybrid rapeseed is easy to realize industrialization from seed production and form a seed brand; (2) yellow commercial rapeseed has obvious characteristics and is easy to purchase and implement High quality and high price; (3) The product characteristi...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 李加纳谌利柴友荣梁颖
Owner SOUTHWEST UNIVERSITY
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