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Method for producing xylitol and arabitol simultaneously by utilizing xylose mother liquid

A technology of arabinitol and xylose mother liquor, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of low utilization rate of xylose and low conversion rate of xylitol, and achieve high conversion rate Effect

Active Publication Date: 2013-04-03
山东绿健生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Patent CN 101643752A introduces a method for producing xylitol and L-arabinose by using xylose mother liquor, converting xylose in xylose mother liquor into xylitol by yeast fermentation, and extracting L-arabinose by chromatographic separation , but due to the low conversion rate of yeast fermenting xylose into xylitol, the utilization rate of xylose in the xylose mother liquor is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Take 1000 g of xylose mother liquor with a mass concentration of 65%, and its composition is: xylose 52.53%, arabinose 25.56%, glucose 14.97%, and galactose 3.483%. Dilute with desalinated water to a mass concentration of 32%, decolorize and filter with activated carbon and purify and remove impurities by ion exchange; add yeast extract with 2% xylose mother liquor mass, 1% magnesium sulfate, and 0.5% dipotassium hydrogen sulfate to adjust the pH value 4.5, sterilized, then insert the yeast seed solution according to the inoculum amount of 8%, and ferment for 20 hours at a temperature of 30°C, an aeration rate of 0.4vvm, and a stirring speed of 130r / min to obtain a fermented liquid; the fermented liquid was first plated Frame filtration, and then sterilized by metal microfiltration with a molecular weight cut-off of 10,000 to 1,000,000, then decolorized, filtered, and ion-exchanged to remove impurities, and initially concentrated to a mass concentration of 50%...

Embodiment 2

[0041]Example 2: Take 1000 g of xylose mother liquor with a mass concentration of 65%, and its composition is: xylose 52.53%, arabinose 25.56%, glucose 14.97%, and galactose 3.483%. Dilute with desalinated water to a mass concentration of 25%, decolorize and filter through activated carbon and purify and remove impurities by ion exchange; add yeast extract with 1% xylose mother liquor quality, 0.5% peptone, 0.2% corn steep liquor, 0.5% magnesium sulfate, 0.5% dipotassium hydrogen sulfate, 0.01% sodium dihydrogen sulfate, adjust the pH value to 3.5, sterilize, then insert the yeast seed solution according to the inoculum amount of 5%, at a temperature of 28°C and an air flow of 0.2vvm 1. Ferment for 10 hours under the condition of stirring speed of 100r / min to obtain the fermented liquid; filter the fermented liquid with plate and frame firstly, then pass through metal microfiltration with a molecular weight cut-off of 10,000 to 1,000,000 to sterilize bacteria, then go through d...

Embodiment 3

[0042] Example 3: Take 1000 g of xylose mother liquor with a mass concentration of 65%, and its composition is: xylose 52.53%, arabinose 25.56%, glucose 14.97%, and galactose 3.483%. Dilute with desalinated water to a mass concentration of 40%, decolorize and filter through activated carbon and purify and remove impurities by ion exchange; add 4% ammonium sulfate, 1% beef extract, and 2% disodium bisulfate to adjust the pH value of the xylose mother liquor 5.5, sterilized, then insert the yeast seed solution according to 10% inoculum amount, and ferment for 28 hours under the conditions of temperature 35°C, ventilation rate 0.5vvm, and stirring speed 150r / min to obtain fermentation liquid; Frame filtration, and then sterilized by metal microfiltration with a molecular weight cut-off of 10,000 to 1,000,000, then decolorized, filtered, and ion exchanged to remove impurities, and initially concentrated to a mass concentration of 55%; adjust the pH of the initially concentrated sug...

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Abstract

The invention discloses a method for producing xylitol and arabitol simultaneously by utilizing xylose mother liquid, which comprises the following steps: (1) diluting the xylose mother liquid, and obtaining a purifying liquid after decolorizing filtration and ion exchange; (2) adding nutrients into the purifying liquid, sterilizing, and then inoculating yeast and fermenting; (3) filtering and sterilizing the fermenting liquid, then carrying out the decolorizing filtration and the ion exchange, and carrying out initial concentration; (4) allowing the concentrated liquid to be prepared into mixed sugar alcohol by catalytic hydrogenation; (5) carrying out chromatographic separation for the mixed sugar alcohol after the decolorizing filtration and the ion exchange to obtain two fractions which are respectively a xylitol fraction and an arabitol fraction; and (6) carrying out evaporation concentration and crystallization for the two fractions so as to obtain xylitol crystals and arabitol crystals. The invention converts xylose and arabinose into xylitol and arabitol by hydrogenation, the conversion rate is higher compared with the method for producing xylitol by yeast fermentation.

Description

technical field [0001] The invention relates to a method for simultaneously producing xylitol and arabitol by using xylose mother liquor, and belongs to the field of biotechnology. Background technique [0002] Xylitol is a kind of pentitol, the sweetness is similar to that of sucrose, because it has anti-caries, will not cause blood sugar level rise, lose weight and other functions, so it is widely used in the food industry as a sweetener, and as a cure for diabetes and hepatitis Clinical nutritional and therapeutic agents for patients are widely used in the field of medicine. In addition, as an important chemical raw material, it is widely used in national defense, leather, plastics and coatings. Arabitol is also a pentitol, an isomer of xylitol, and has similar physiological functions to xylitol, but due to the current limited production and high price, it cannot be used in many fields like xylitol be utilized. [0003] Xylose mother liquor is a by-product obtained aft...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C31/18C07C29/141
Inventor 信成夫景文利任尚美
Owner 山东绿健生物技术有限公司
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