Method for preparing ferulic acid by utilizing corn bran hydrolyzed by multifunctional enzyme

A multifunctional enzyme and ferulic acid technology, applied in the field of corn deep processing, can solve the problems of low hydrolysis rate, achieve energy saving, simple process, and increase added value

Inactive Publication Date: 2010-09-29
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ferulic acid is a kind of phenolic acid. On the plant cell wall, due to the cross-linking of ferulic acid, the polysaccharides of the cell wall are closely combined. The single use of a certain polysaccharide hydrolase cannot penetrate into the polysaccharide molecules well. , resulting in too low a hydrolysis rate

Method used

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  • Method for preparing ferulic acid by utilizing corn bran hydrolyzed by multifunctional enzyme
  • Method for preparing ferulic acid by utilizing corn bran hydrolyzed by multifunctional enzyme
  • Method for preparing ferulic acid by utilizing corn bran hydrolyzed by multifunctional enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Add 650L of 0.6% NaOH to 100kg of 80-mesh corn husks and soak in dark for 1.5 hours;

[0028] 2) Sterilize the soaked material under high pressure at 0.105PKa at 121°C for 30-60min;

[0029] 3) After cooling the soaking solution to 50°C, adjust the pH to 8, add 20ul of multifunctional enzyme (APE1547) per ml of soaking solution to hydrolyze the ester bond and peptide bond on the cell wall, and put it in a shaker at 50°C for 2 hours. ; Centrifuge at 5500r for 10min;

[0030] 4) Take the supernatant, extract it with ethyl acetate, and measure it by gas chromatography. Gas chromatography detection conditions: column temperature 80°C-210°C, heating rate: 15°C / min, stay at 210°C for 3min. The temperature of the sampling chamber is 240°C , The detection chamber temperature is 240°C. The chromatographic column is: DB1701. see results figure 1 .

Embodiment 2

[0032] 1) Add 70 L of 0.6% NaOH to 10 kg of 80-mesh corn husks, and soak for 1.5 hours in the dark;

[0033] 2) Sterilize the soaked material under high pressure at 0.105PKa at 121°C for 30-60min;

[0034] 3) After cooling the soaking solution to 50°C, adjust the pH to 8, add 20ul of multifunctional enzyme (APE1547) per ml of soaking solution to hydrolyze the ester bond and peptide bond on the cell wall, and put it in a shaker at 50°C for 2 hours. ; Centrifuge at 5500r for 10min;

[0035] 4) Take the supernatant, extract it with ethyl acetate, and measure it by gas chromatography. Gas chromatography detection conditions: column temperature 80°C-210°C, heating rate: 15°C / min, stay at 210°C for 3min. The temperature of the sampling chamber is 240°C , The detection chamber temperature is 240°C. The chromatographic column is: DB1701. see results figure 2 .

Embodiment 3

[0037] Purification of ferulic acid

[0038] The supernatant from Step 3 of Example 1, pH = 9.0, was subjected to column chromatography using HPD-600, the ratio of the column material to the supernatant was 1:6 (W / V), sealed and placed at 50°C, 110r / min Complete adsorption in constant temperature shaker, see image 3 ;

[0039] Elution, the eluent is: concentrated hydrochloric acid: water: ethanol 4:35:61, the eluate is concentrated, extracted with an equal volume of ethyl acetate, detected by gas chromatography, see Figure 4 .

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Abstract

The invention provides a method for preparing ferulic acid by utilizing corn barn hydrolyzed by a multifunctional enzyme. The method comprises the following steps of: adding the corn bran into NaOH and soaking darkly, sterilizing at high pressure, adding the multifunctional enzyme with peptidase and esterase activities for hydrolyzing ester bonds and peptide bonds on the cell wall, centrifugallytaking supernatant, treating the supernatant by adopting a column chromatography and eluting, concentrating eluent, extracting by adopting ethyl acetate and rotatably evaporating to obtain the ferulic acid. The ferulic acid is produced by utilizing the corn bran as a by-product in the process of grain processing or fuel ethanol production and the hydrolysis of the multifunctional enzyme with peptidase and esterase activities, therefore, the invention has simple process, time and energy saving and increased additional value of the corn by-products.

Description

technical field [0001] The invention relates to a production method of ferulic acid, in particular to a method for preparing ferulic acid by hydrolyzing corn husks with a multifunctional enzyme. The multifunctional enzyme expressed by engineering bacteria is used to obtain ferulic acid, which belongs to the technical field of corn deep processing. Background technique [0002] At present, corn bran is a by-product of corn deep processing. Corn bran not only contains rich polysaccharides, proteins, carbohydrates, vitamins and minerals, but also contains more phenolic acids or phenolic compounds. Ferulic acid is a kind of phenolic acid. On the plant cell wall, due to the cross-linking of ferulic acid, the polysaccharides of the cell wall are closely combined. The single use of a certain polysaccharide hydrolase cannot penetrate the polysaccharide molecules well. , causing the hydrolysis rate to be too low. If the multifunctional hydrolase can be used to act on the cell wall a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42
Inventor 张桂荣费瑞程英琨牛砚涛宋月爽
Owner JILIN UNIV
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