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Bacillus firmus and application thereof

A technology of Bacillus firmus, bf-02cgmccno.3129, applied in the field of microbiology

Inactive Publication Date: 2012-01-11
宁国市百立德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, prevent other pests and diseases such as: cucumber powdery mildew, walnut anthracnose, cucumber gray mold, wheat sheath blight, apple ring spot, tobacco red spot, corn small spot, winter jujube black spot, cotton wilt, cotton Red rot, wheat head blight, etc. have not been reported

Method used

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  • Bacillus firmus and application thereof
  • Bacillus firmus and application thereof
  • Bacillus firmus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1. Isolation and purification of Bacillus firmus Bf-02 CGMCC No.3129

[0014] Bacillus firmus (Bacillus firmus) Bf-02 CGMCC No.3129 of the present invention is obtained from the soil of the experimental nursery of Shandong Academy of Forestry Sciences using a soil dilution separation method. The separation method is:

[0015] (1) Soil sample collection: sample 200g from the soil surface to 15cm, take several samples at one location, mix them through a coarse sieve, and take 25g for separation;

[0016] (2) Culture medium plate preparation: NA medium (nutrient agar medium) and PDA (potato dextrose agar medium) medium are melted, pour into sterilized culture dish respectively, cool, make NA and PDA plate; The formula of NA medium is peptone 10g, beef extract 3g, NaCl 5g, agar 14g, distilled water 1000mL, pH7.3. The formula of the PDA medium is 200 g of potatoes (peeled and cut into pieces), 17 g of glucose, 12-14 g of agar, and 1000 mL of distilled water.

[0017] (3) W...

Embodiment 2

[0025] Embodiment 2: Fermentation of Bacillus firmus (Bacillus firmus) Bf-02 CGMCC No.3129

[0026] NA medium formula (commercially available): 10g peptone, 3g beef extract, 5g NaCl, 14g agar, 1000 mL distilled water, pH7.3.

[0027] NB formula: peptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1000mL, pH7.3;

[0028] NYBD formula: beef extract 8g, yeast extract 5g, glucose 10g, sodium chloride 5g, water 1000mL, pH 7.3.

[0029] A large amount of fermentation culture formula (mass percentage content): 1.8% bean flour, 1.6% corn flour, 0.44% starch, 0.44% calcium carbonate, 0.47% fish meal, 0.01% magnesium sulfate, 0.03% potassium dihydrogen phosphate, and the rest is water, pH 7.3.

[0030] 1. Bacillus firmus (Bacillus firmus) Bf-02 CGMCC No.3129 strain activation

[0031] Inoculate the strains preserved on the slant onto the slant of the NA test tube and incubate at 28°C for 24h.

[0032] 2. Preparation of Bacillus firmus Bf-02 CGMCC No.3129 seed solution ...

Embodiment 3

[0038]Embodiment 3: the application of Bacillus firmus (Bacillus firmus) Bf-02 CGMCC No.3129 fermented liquid

[0039] In order to clarify the yield-increasing effect of Bacillus firmus (Bacillus firmus) Bf-02 CGMCC No.3129 fermentation broth on the control of gray mold of cucumber, and to provide a basis for its popularization and application. We conducted this test at the Linyi test base from January to February 2008, and the test results are reported as follows:

[0040] 1 Test materials and methods

[0041] 1.1 Drugs to be tested

[0042] Bacillus firmus (Bacillus firmus) Bf-02 CGMCC No.3129 fermented liquid produced by the fermentation method of the present invention; 40% imimethamine wettable powder (produced by Shandong Shouguang Shuangxing Pesticide Co., Ltd., commercially available).

[0043] 1.2 Test crops and control objects: the test crops are cucumbers, and the variety is Changchun Mici; control objects: Botrytis cinerea

[0044] 1.3 Conditions of the test site...

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PUM

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Abstract

The invention discloses a bacillus firmus strain. The bacillus firmus strain is bacillus firmus Bf-02 CGMCC No.3129, and can prevent and control a plurality of plant diseases, such as the anthracnose of walnuts, the gray mold of cucumber, wheat sharp eyespots, the ring rot of apples, tobacco brown spots, corn southern leaf blight, Dongzao jujube black patch, cotton fusarium wilt, cotton red rot, wheat scab and the like. Tests prove that the zymotic fluid of the bacillus firmus Bf-02 CGMCC No.3129 has remarkable effect of preventing and controlling vegetables, particularly the gray mold of the cucumber with the healthy growth of the vegetables and without hazards, and also remarkable growth promotion and yield increasing effects on the cucumber with the growth rate of 29.95 percent after one-time application.

Description

technical field [0001] The invention relates to a strain of Bacillus firmus and application thereof, belonging to the field of microorganisms. Background technique [0002] The planting industry causes huge losses due to pests and diseases every year. There are many ways to control pests and diseases, and chemical control plays an important role in the history of agricultural development in the world. For centuries, chemical pesticides have played a very important role in the control of pests and diseases in agricultural and forestry production. However, due to extensive use, serious environmental pollution has been caused, and the resistance of pests and diseases has increased, forming a vicious circle and seriously affecting human health and ecological balance. Biological control is an inevitable trend in agricultural and forestry production. The use of antagonistic microorganisms to control plant diseases is one of the main contents. Among the various bio-control bact...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00A01P5/00A01P7/04A01P21/00C12R1/07
Inventor 牛赡光王清海刘幸红张淑静
Owner 宁国市百立德生物科技有限公司
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