Process for extracting anthocyanin from blueberries
A technology of anthocyanin and blueberry, which is applied in the field of extracting anthocyanin, can solve the problems of low industrialization and high energy consumption, and achieve the effects of increasing content and color-price ratio, ensuring yield, and increasing adsorption capacity
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Embodiment 1
[0028] Take 10kg of blueberries and crush them, put them into a multi-functional extraction tank, add 50Lph1 hydrochloric acid aqueous solution, soak for 2 hours, filter the liquid, then add 30ph2L hydrochloric acid aqueous solution to soak for 2 hours, after suction filtering the liquid, continue to add 30L ph3 hydrochloric acid aqueous solution to soak for 2 hours, Suction filter the liquid, combine the three extracts and add 320ml of prepared ZTC-III fruit juice clarifier, stir, mix and precipitate for 4 hours, filter and add 2LX-5 macroporous resin column for adsorption, flow rate 15L / h, after the adsorption is saturated, wash with deionized water To sugar-free color, then elute with 12L of 70% ethanol solution, collect the eluate, add a hollow composite membrane with a molecular weight cut-off of 3000 for ultrafiltration, control the inlet pressure within the range of 0.3-0.4Mpa, collect the permeate and then add a molecular weight cut-off of 200 Concentrate, collect conce...
Embodiment 2
[0030] Take 10kg of blueberries and crush them, put them into a multi-functional extraction tank, add 50Lph2 citric acid aqueous solution, soak for 4 hours, filter the liquid, then add 50Lph2L citric acid aqueous solution for 4 hours, filter the liquid, combine the extracts and add the prepared ZTC- III Juice clarifier 300ml, stirring and mixing for 6 hours. The filtered extract was added to a 2LD101 macroporous resin column for adsorption at a flow rate of 10L / h. After the adsorption was saturated, it was washed with deionized water to a sugar-free color, and then eluted with 14L of 70% ethanol solution. Filtration, the inlet pressure is controlled within the range of 0.3-0.4Mpa, the permeate is collected and added to a hollow composite membrane with a molecular weight cut-off of 200 for nanofiltration concentration, the concentrated solution is collected, and spray-dried to obtain 60g of anthocyanin product with a content of 82%.
Embodiment 3
[0032] Take 10kg of blueberries and crush them, put them into a multifunctional extraction tank, add 50Lph2 sulfuric acid aqueous solution, soak for 4 hours, add 100ml of prepared ZTC-III juice clarifier to the filtered liquid, mix and settle for 4 hours, then add 50Lph2L sulfuric acid to the extraction tank Soak in the aqueous solution for 4 hours, filter the liquid with suction, add 80ml of prepared ZTC-III fruit juice clarifier, stir, mix and precipitate for 4 hours, filter the extract and add it to a 2LX-5 macroporous resin column for adsorption at a flow rate of 10L / h. After the adsorption is saturated, remove Wash with ionized water until sugar-free, then elute with 14L 70% ethanol solution, collect the eluate, add hollow composite membrane ultrafiltration with a molecular weight cut-off of 3000, control the inlet pressure within the range of 0.3-0.4Mpa, collect the permeate and add the molecular weight cut-off Concentrate by nanofiltration with a hollow composite membran...
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