Biological preparation method of Hypericum perforatum L extractive

Abstract

The invention relates to a biological preparation method of a Hypericum perforatum L extractive, providing a preparation method which can utilize medicinal material resources adequately, has simple process, is suitable for large-scale production and can prepare the Hypericum perforatum L extractive in accordance with or higher than international standards. The method comprises the following stepsof: drying the Hypericum perforatum L firstly and smashing into Hypericum perforatum L powder, then weighing enzyme, adding warm water in the enzyme to dissolve the enzyme, activating enzyme solutionby water bath, mixing the Hypericum perforatum L powder with water, performing water bath, soaking, cooling, adjusting pH with hydrochloric acid, stirirng uniformly, heating by water bath, adding theactived enzyme solution, mixing uniformly, performing enzymolysis, filtering, abandoning the filter to obtain medicinal material powder after enzymolysis, adding ethanol and fluxing for extraction, filtering, combining the filtrate, condensing under reduced pressure, recycling ethanol, and drying to obtain the Hypericum perforatum L extractive. The invention improves the content of hypericin in the extractive and the abandoned filtrate after enzymolysis takes away a large amount of water-soluble impurities so that the moisture of the extractive is not needed to absorb again. The biological method is safe, pollution free and has simple operation and is easy for industrialized application.

Description

1. Technical field [0001] The invention relates to the field of medicine, in particular to a biological preparation method of Hypericum perforatum extract. 2. Background technology [0002] Hypericin (chemical name: 4,4,5,5,7,7-hexahydroxy-2,2-dimethyl-meso-naphtho) contained in Hypericum Perforatum L. Dianthrone) has excellent biological activities, such as antidepressant, antiviral, anticancer and photodynamic activity, especially for a series of neurological disorders such as depression and sub-health symptoms caused by tension. The medical profession pays attention. The increasing demand for Hypericum perforatum extract in the international market has also stimulated the production of Hypericum perforatum extract in my country in recent years. [0003] However, the content of hypericin in the whole plant of Hypericum perforatum is only a few ten-thousandths. Therefore, how to prepare qualified (the 24th edition of the United States Pharmacopoeia and the import and expo...

AI Technical Summary

Problems solved by technology

As we all know, the disadvantage of lye extraction is that the color of the obtained extract is poor, and a large amount of NaCl is entrained in the product, which brings a lot of inconvenience to the purification

[0006] 3. Organic solvent extraction-adsorption material enrichment and purification method: Chinese patent 200610078988.9 discloses an extraction method of hypericin: Hypericum perforatum is extracted with ethanol, the filtrate is concentrated under reduced pressure, and the concentrated solution is adsorbed with resin, eluted, collected The eluate is concentrated under reduced pressure to obtain a hypericin extract. The product obtained by this method contains higher hypericin, but the process is relatively complicated and the cost is high, and the resin used for adsorption is a chemically synthesized macromolecular compound , which contain unsynthesized chemical monomers, such as benzene, divinylbenzene, toluene, xylene and other organic residues, are not easy to be used for food and consumption, because the State Food and Drug Administration has restricted the separation and purification of macroporous adsorption resins. There are strict regulations on organic residues in health food and raw materials prepared with styrene-based resins. Therefore, the method of purifying Hypericum perforatum extract with macroporous adsorption resins is largely limited, and it is difficult to achieve industrialized mass production. Requirements, currently mainly still in the laboratory stage

[0007] Since the active ingredients in plants usually exist in plant cells, the use of biological enzymes to destroy the cell wall can promote the dissolution of active ingredients, thereby increasing the content of active ingredients in the extract and improving the utilization of medicinal materials, but so far no public reports have been seen. Application of enzymatic hydrolysis in the preparation of Hypericum perforatum extract