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Method for staining plant tissue section by using thionine

A plant tissue and slice dyeing technology, applied in the field of plant slice dyeing, can solve the problems of single color, influence of observation effect, inability to distinguish different tissues, etc., and achieve intuitive effect

Inactive Publication Date: 2010-09-01
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The commonly used staining agents for freehand section staining of plant tissues are 0.5% safranin and 0.2% fast green. Both staining agents can only make the plant tissue and structure present a single color, and cannot distinguish different tissues according to the color. Observe the effect affected

Method used

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  • Method for staining plant tissue section by using thionine
  • Method for staining plant tissue section by using thionine
  • Method for staining plant tissue section by using thionine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) 30 milliliters of thionine solutions with a mass fraction of 0.2% are prepared, and added to a petri dish for use;

[0016] (2) Get the root of Chlorophytum chinensis with root hairs, slice it by hand and store it in a petri dish filled with clear water;

[0017] (3) Dip the plant tissue section soaked in clear water in (2) with a brush, and transfer to the petri dish containing the thionine solution in (1) for staining for 3 minutes;

[0018] (4) Dip the stained plant tissue section in (3) with a brush, transfer it to a glass slide, and drop a drop of 0.5% NaHCO on the section 3 Solution, cover the cover glass, gently press the cover glass with tweezers, then absorb excess water around the cover glass with absorbent paper, observe and take pictures under the microscope.

[0019] As shown in Figure 1, the root hair cells and epidermal cells of the root of Chlorophytum are purple, the parenchyma cells of the cortex are mostly light pink, the endothelial cells are li...

Embodiment 2

[0021] (1) 30 milliliters of thionine solutions with a mass fraction of 0.5% are prepared, and added to a petri dish for use;

[0022] (2) Get the stem of Euonymus microphylla, cut the plant material by free-hand slicing, and store the slices in a petri dish filled with clear water for later use;

[0023] (3) Dip the plant tissue section soaked in clear water in (2) with a brush, and transfer to the petri dish containing the thionine solution in (1) for staining for 5 minutes;

[0024] (4) Dip the stained plant tissue section in (3) with a brush, transfer it to a glass slide, and drop a drop of 1% NaHCO on the section 3 Solution, cover the cover glass, gently press the cover glass with tweezers, then absorb excess water around the cover glass with absorbent paper, observe and take pictures under the microscope.

[0025] As shown in Figure 2, the cell wall of the stem epidermal cells of Euonymus microphylla is blue, the epidermal hairs are colorless, the vessels are blue, and ...

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Abstract

The invention relates to a method for staining a plant section, which belongs to the technical field of chemical staining agent application. The method comprises the following steps of: staining a plant tissue section by using 0.2 to 0.5 percent thionine aqueous solution; loading and performing microscopical study; and distinguishing different plant tissues and structures according to different colors. The method for staining the plant tissue section has the characteristics of rapidness, simplicity and convenience, and intuitional effect.

Description

technical field [0001] The invention relates to a method for dyeing plant slices, and belongs to the technical field of chemical dye application. Background technique [0002] The commonly used staining agents for freehand section staining of plant tissues are 0.5% safranin and 0.2% fast green. Both staining agents can only make the plant tissue and structure present a single color, and cannot distinguish different tissues according to the color. Observe the effect affected. Contents of the invention [0003] The object of the present invention is to provide a method for dyeing plant tissue slices, which can make plant tissues present different colors, and different plant tissues and structures can be distinguished according to the colors, which is fast, simple and intuitive. [0004] The technical scheme adopted in the present invention is: [0005] The method for staining plant tissue sections with thionine comprises the steps: [0006] (1) Prepare 30 milliliters of t...

Claims

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Application Information

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IPC IPC(8): G01N1/30
Inventor 王长泉
Owner SHANDONG UNIV OF TECH
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