Novel technique for extracting L-tryptophan from fermentation broth
A technology for tryptophan and fermentation broth, applied in the new technological field of separating and extracting L-tryptophan, can solve the problems of easy product deterioration, environmental pollution, low extraction yield, etc., achieve thorough sterilization and impurity removal, and improve extraction Yield, effect of short filtration time
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Embodiment 1
[0024] The first step is to pretreat the L-tryptophan-containing fermentation broth produced by the direct fermentation method: the treatment method is to add 0.1% alum and 0.1% NaHSO in the volume of the fermentation broth, stir and heat to 60 ° C for 30 minutes to produce treatment fluid;
[0025] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3Top wash with purified water until the tryptophan content in the concentrated liquid is lower than 1.0g / L, then stop the machine, and obtain the tryptophan microfiltrate and bacterial protein from which the bacteria are removed. The bacteria removal rate reaches 99.74%, and the L-tryptophan The yield is 99....
Embodiment 2
[0033] The first step, the fermentation broth containing L-tryptophan produced by the direct fermentation method is pretreated: the treatment method is to add 0.1% alum and 0.1% NaHSO in the volume of the fermentation broth, stir and heat to 60 ° C for 30 minutes to prepare treatment fluid;
[0034] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3 Top wash with purified water until the tryptophan content in the concentrated liquid is lower than 1.0g / L, then stop the machine, and obtain the tryptophan microfiltrate and bacterial protein from which the bacteria are removed. The bacteria removal rate reaches 99.74%, and the L-tryptophan The yield is 99...
Embodiment 3
[0042] The first step, the fermentation broth containing L-tryptophan produced by the direct fermentation method is pretreated: the treatment method is to add 0.1% alum and 0.1% NaHSO in the volume of the fermentation broth, stir and heat to 60 ° C for 30 minutes to prepare treatment fluid;
[0043] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3 Top washing with purified water until the tryptophan content in the dope is lower than 1.0g / L, then shut down. Obtain tryptophan microfiltrate and bacterial protein from which the bacteria were removed; the bacteria removal rate reached 99.74%, and the yield of L-tryptophan was 99.3%;
[0044] The third s...
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