Production method of adiantum

Abstract

The invention relates to the technical field of plant tissue culture, in particular to a production method of adiantum, which comprises the following steps: A taking adiantum sporophyl as the explant; B preparing an M1 culture medium for the spore germination and the proliferation and subculture of prothallium; C germinating the spore, transferring the explant onto the M1 culture medium after sterilizing the surface of the explant and putting the M1 culture medium in a culturing room for culture; D proliferating and subculturing the prothallium, dividing the prothallium group generated after the spore is germinated into small blocks, and transferring the small blocks to a new M1 culture medium for proliferation and subculture; and E inducing the sporophyte, taking the prothallium blocks, mixing the prothallium blocks with a blending solution, grinding the mixture by a soymilk grinder, spraying the ground mixed liquid to a sieve tray provided with matrix, and putting the sieve tray in a greenhouse for cultivating to obtain the adiantum sporophyte plant. The invention utilizes incomplete tissue culture to produce the adiantum, thereby reducing the generation of subculture, reducing the production cost, reducing the mutation rate, increasing the production efficiency and saving the manpower.

Description

Technical field: [0001] The invention relates to the technical field of plant tissue culture, in particular to a production method of Adiantum. Background technique: [0002] Adiantum, also known as wire grass, Adiantum, and water pig hair soil, is a perennial herbaceous foliage plant of the Adiantum family Adiantum. Its stems and leaves are beautiful and varied, graceful in shape, and small in size. It can be used as cut-leaf flower arrangement material, bonsai ornamental, or as a local plant. It is deeply loved by Europeans, Americans, and Japanese. In recent years, it has also developed rapidly in the domestic market. [0003] At present, in the production of ferns, most of them use tissue culture to induce GGB or prothallium first, and then use GGB or prothallium to induce sporophyte plants, but Adiantum can only obtain a large number of Prothallus, it is difficult to obtain sporophytic plants; although a large number of plants can be obtained by sowing and propagating ...

AI Technical Summary

Problems solved by technology

[0003] At present, in the production of ferns, most of them use tissue culture to induce GGB or prothallium first, and then use GGB or prothallium to induce sporophyte plants, but Adiantum can only obtain a large number of Prothallus, it is difficult to obtain sporophytic plants; although a large number of plants can be obtained by sowing and propagating with spores, the spores are easy to compete with fungi, algae or mosses for nutrients when sowing and reduce the germination rate and growth rate, and the spores are stored. The germination rate of spores and the induction rate of sporophytes are greatly reduced, which affects the annual supply of Adiantum fern. In addition, direct seeding with spores has stricter environmental requirements, and the light and humidity of greenhouses are unstable. Death due to adversity; spore sowing density is difficult to control, and the sown prothallus needs to be transplanted to produce sporophytes, which will inevitably increase manpower