HCV (Hepatitis C Virus) antibody detection kit as well as preparation method and detection method thereof

Abstract

The invention relates to a HCV (Hepatitis C Virus) antibody detection kit based on a flowing microsphere carrier technology as well as a preparation method and a detection method thereof. The preparation method and the detection method comprise the following steps of: coating activated high molecular polymer microspheres on HCV recombinant antigen, enclosing blank combination points by using bovine serum albumin, and preparing specific HCV probe-high molecular polymer microspheres; co-culturing with a specimen to be tested and capturing HCV antibodies to the tested; washing and centrifugally removing combined HCV antibodies, and then adding fluorescently-labeled anti-human IgG or IgM antibodies; detecting the fluorescence intensity of the microspheres by using a flow cytometry and indirectly carrying out qualitative and quantitative analysis on the tested antibodies. The kit has high sensitivity, good specificity and good stability and can carry out multivalence detections including HCV antibodies simultaneously.

Description

technical field [0001] The invention relates to a hepatitis C virus (HCV) antibody test kit based on flow microsphere carrier technology and a preparation method and a detection method thereof, which belong to the technical field of immunoanalysis medical diagnosis. Background technique [0002] Hepatitis C virus (hepatitis C for short) is one of the infectious diseases that seriously threaten human health. Currently, about 170 million people are infected worldwide, with 3 to 4 million new cases every year. Among them, the infection rate and incidence rate are particularly high in developing countries. The infection rate of hepatitis C in my country is about 3.2%, and there are at least 40 to 60 million hepatitis C patients and carriers of hepatitis C virus. [0003] Hepatitis C is a liver disease that is transmitted through blood, sexual contact, mother to child and daily life contact. The characteristics are: the symptoms of acute hepatitis are mild, often without jaund...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a hepatitis C virus antibody detection kit based on flow microcarrier technology and its preparation and detection method, which solves the shortcoming of single function in the prior art, and can detect multivalent antibodies including HCV , which can be detected qualitatively and quantitatively, and has the characteristics of high sensitivity, high specificity and high stability