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Rapid detection method for chili (sweet) pepper root rot diseases

A detection method and root rot technology, applied in the biological field, can solve problems such as hidden dangers in pepper production

Inactive Publication Date: 2010-06-30
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

Since 2004, Capsicum Phytopathora Root Rot has generally occurred in Shandong, Liaoning, Beijing, Hebei and other major pepper producing areas in northern my country, which is a devastating disease caused by Phytophthora capsici. In the absence of knowledge of the pathogen, local vegetable farmers have adopted the traditional method of prevention and control of root rot caused by Fusarium fungus, but with little success. The disease has become a "cancer" in local pepper production
There are few researches on the detection methods of pepper root rot disease at home and abroad. Our country has not yet established a rapid and effective detection method for this disease, which has left a major hidden danger for the production of peppers in our country. Therefore, a rapid and accurate detection technology has been established. The method is imminent and significant

Method used

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  • Rapid detection method for chili (sweet) pepper root rot diseases
  • Rapid detection method for chili (sweet) pepper root rot diseases
  • Rapid detection method for chili (sweet) pepper root rot diseases

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Experimental program
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Embodiment Construction

[0052] Through the optimization of Dot-ELISA rapid detection conditions for pepper root rot and cucumber wilt, a kit for the rapid diagnosis of these two diseases in the field was developed. The accuracy of field prediction of diseases reached 95%, and the sensitivity reached 95%. %.

[0053] 1. Field rapid detection technology of pepper root rot blight

[0054] Determination of optimal working conditions for indirect Dot-ELISA:

[0055] According to the indirect Dot-ELISA method, the working concentration of the antibody was determined to be 160 times (8.69 μg / mL). The best incubation time for antigen and antibody is 1.5h to 2.0h. The optimal working concentration of the enzyme-labeled secondary antibody is 600×. According to the indirect Dot-ELISA optimization reaction procedure established above, the optimal incubation time of the enzyme-labeled secondary antibody was determined to be 1.5h.

[0056] 2. Summary

[0057] Using nitrocellulose membrane as solid phase carri...

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Abstract

The invention provides a rapid detection method for chili (sweet) pepper root rot diseases, which belongs to the field of biotechnology. The invention relates to the rapid detection method for chili (sweet) pepper root rot diseases, which is characterized in that the method applies an improved novel technique of ELISA, namely a Dot-ELISA technique, is low in consumption of reagents, simple and fast to operate, free from need for special equipment, applicable to basic units, long in preservation period of antigen membrane; 20 percent of antigen membrane can be preserved for half an year without affecting the activity of the antigen membrane; the antigen membrane can be mailed for on-site epidemiological investigation; detection result can be kept for a long time to provide convenience for reexamination; and the method is suitable for on-site detection and the rapid prediction of the epidemic occurrence of diseases. The invention also relates to detection programs, working conditions, detectable measurement, medicaments and appliances used for detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a rapid detection method for an important soil-borne fungal disease in pepper (sweet) pepper production. Background technique [0002] In recent years, with the increase of protected vegetable cultivation area in my country, it is difficult to rotate crops and stubble, and soil-borne diseases are becoming more and more serious. Since 2004, Capsicum Phytopathora Root Rot has generally occurred in Shandong, Liaoning, Beijing, Hebei and other major pepper producing areas in northern my country, which is a devastating disease caused by Phytophthora capsici. In the absence of knowledge of the pathogen, local vegetable farmers have adopted the traditional method of prevention and control of root rot caused by Fusarium fungus, but with little success. The disease has become a "cancer" of local pepper production. The prevention and control of capsicum root rot disease focuses on rapid and accu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/531G01N33/535
Inventor 石延霞李宝聚朱辉谢学文
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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