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Method for preparing genistein from fructus sophorae

A technology of genistein and sophora japonica, which is applied in the direction of fermentation, etc., can solve the problems of difficult operation, high equipment requirements, and low industrialization level, so as to improve product yield and content, low equipment requirements, and easy control The effect of reagent carryover

Inactive Publication Date: 2010-06-30
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing process for producing genistein is difficult to operate, requires a high degree of equipment, and the degree of industrialization is not high

Method used

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  • Method for preparing genistein from fructus sophorae

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Effect test

Embodiment 1

[0032] Sophora japonica was crushed to 30 mesh, 10 kg (sophoricroside content 9.5%) was taken, 20 L of water was added, the pH was adjusted to 5, 200 g of cellulase was added, heated to 50° C., and incubated for 10 hours. Add 60L of 90% ethanol, reflux and extract for 2 hours, extract twice, combine the extracts and concentrate under reduced pressure to 70% alcohol concentration, leave to crystallize for 8 hours, filter the crystals, add 5 times their weight in hot water at 80°C, stir and wash, filter to obtain the dye Crude lignin. The crude genistein was saturated and dissolved with 95% ethanol under reflux, adjusted the pH to 4, left to crystallize for 12 hours, filtered to obtain crystals, added 50% ethanol twice the weight of the crystals, stirred and washed, filtered, and dried to obtain 360 grams of finished product with a content of 99 %.

Embodiment 2

[0034] Sophora japonica was crushed to 60 mesh, 10 kg (sophoricroside content 9%) was taken, 10 L of water was added, the pH was adjusted to 5.5, 100 g of β-glucosidase was added, heated to 45° C., and incubated for 12 hours. Add 80L of 90% ethanol, reflux and extract for 1 hour, extract twice, combine the extracts and concentrate under reduced pressure to an alcohol concentration of 65%, leave to crystallize for 12 hours, filter the crystals, add 6 times their weight in hot water at 70°C, stir and wash, and filter to obtain the dye Crude lignin. The crude genistein was saturated and dissolved with 93% ethanol under reflux, adjusted the pH to 4.5, left to crystallize for 14 hours, filtered to obtain crystals, added 70% ethanol twice the weight of the crystals, stirred and washed, filtered, and dried to obtain 330 grams of finished product with a content of 98 %.

Embodiment 3

[0036] Sophora japonica was crushed to 40 mesh, 10kg (sophoricroside content 9%) was taken, 10L of water was added, the pH was adjusted to 4.5, 200g of amylase was added, heated to 55°C, and kept for 15 hours. Add 60L of 95% ethanol, reflux and extract for 1 hour, extract three times, combine the extracts and concentrate under reduced pressure to an alcohol concentration of 60%, leave to crystallize for 10 hours, filter the crystals and add 6 times its weight in hot water at 80°C, stir and wash, and filter to obtain genistein Vegetarian crude products. The crude genistein was saturated and dissolved with 90% ethanol under reflux, adjusted to pH 5, left to crystallize for 14 hours, filtered to obtain crystals, added 70% ethanol twice the weight of the crystals, stirred and washed, filtered, and dried to obtain 340 grams of finished product with a content of 98 %.

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Abstract

A method for preparing genistein from fructus sophorae takes fructus sophorae as raw material and includes that: bio-enzyme with quantity of 1-2% of that of the raw material is added for heat-preservation enzymolysis for 10-15 hours at the temperature of 45-55 DEG C, the enzymolysis product is performed with ethanol backflow extraction for 2-3 times, and then decompression concentration and placement crystallization are carried out, hot water scrubbing crystallization is conducted and the backflow dissolving is performed subsequently, finally placement crystallization is carried out while the pH is 4-5. The invention prepares the product with high content, realizes high yield, has low cost, and is easy for scale-up and industrialization.

Description

Technical field: [0001] The invention relates to a method for preparing genistein from fresh Sophora japonica, in particular to a method for preparing genistein by biological enzymolysis. Background technique: [0002] Genistein [0003] Alias: Genistein, Genistein [0004] Chemical name: 5,7-dihydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one; 4′,5,7-trihydroxyisoflavone [0005] Molecular formula: C 15 h 10 o 5 [0006] Molecular weight: 270.24 [0007] Molecular Structure: [0008] [0009] Genistein belongs to flavonoids, has estrogen and anti-estrogen properties, has antioxidant effect, can inhibit the activity of tyrosine protein kinase (PTK), can inhibit the activity of topoisomerase II, and has the ability to induce cell programming death, increase the efficacy of anticancer drugs, and inhibit angiogenesis. It is a potential cancer chemopreventive agent, and its anticancer effect and mechanism have broad application prospects. [0010] Genistein widely e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06
Inventor 刘东锋张翼杨成东
Owner NANJING ZELANG MEDICAL TECH
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