Reagent (kit) for diagnosing/determining amino acid and method for determining concentration of amino acid
An amino acid and reagent technology, applied in biological testing, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of complicated operation methods, poor specificity, and high cost of instruments and equipment
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Embodiment 1
[0044] The amino acid diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0045] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0046] Stabilizer 500mmol / L
[0047] Coenzyme 3mmol / L
[0048] Amino acid oxidase 10000U / L
[0049] Pyruvate oxidase 12000U / L
[0050] Fatty acid synthase 12000U / L
[0051] Sodium bicarbonate 7mmol / L
[0052] Acetyl-CoA 9mmol / L
[0053] Long chain fatty acid 6mmol / L
[0054] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0055] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the amino acid sample to be tested is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is about 0 minutes,...
Embodiment 2
[0058] The amino acid diagnosis / measurement reagent of this embodiment is a double reagent, including:
[0059] Reagent 1
[0060] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0061] Stabilizer 50mmol / L
[0062] Coenzyme 3mmol / L
[0063]Sodium bicarbonate 7mmol / L
[0064] Acetyl-CoA 9mmol / L
[0065] Long chain fatty acid 6mmol / L
[0066] Reagent 2
[0067] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0068] Stabilizer 50mmol / L
[0069] Amino acid oxidase 10000U / L
[0070] Pyruvate oxidase 12000U / L
[0071] Fatty acid synthase 12000U / L
[0072] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0073] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the amino acid sample to be tested to ...
Embodiment 3
[0076] The amino acid diagnosis / measurement reagents of this embodiment are three reagents, including:
[0077] Reagent 1
[0078] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0079] Stabilizer 50mmol / L
[0080] Coenzyme 3mmol / L
[0081] Sodium bicarbonate 7mmol / L
[0082] Acetyl-CoA 9mmol / L
[0083] Long chain fatty acid 6mmol / L
[0084] Reagent 2
[0085] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0086] Stabilizer 500mmol / L
[0087] Pyruvate oxidase 12000U / L
[0088] Fatty acid synthase 12000U / L
[0089] Reagent 3
[0090] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0091] Stabilizer 500mmol / L
[0092] Amino acid oxidase 10000U / L
[0093] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0094] When measuring amino acid concentration, set on the automatic biochemical analyzer: temperature 37°C, r...
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