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Application of photosynthetic bacteria fermentation broth in pesticide and metal combined contamination soil

A composite technology of photosynthetic bacteria and metals, applied in the restoration of polluted soil, bacteria, methods based on microorganisms, etc., can solve the problems of unreported application technology, achieve broad application prospects, wide sources, and reduce bioavailability Effect

Active Publication Date: 2010-06-23
ZHONGBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some literature reports on photosynthetic bacteria degrading pesticide residues or removing heavy metals lead and cadmium (Li Le et al., Journal of Agricultural Environmental Sciences, 2006, 25 (B09); Luo Yuanhua et al., Tianjin Agricultural Sciences, 2009, 2; a pesticide residue Degrading bacteria agent and its production method, ZL 01108446.4; application of Rhodobacter sphaeroides fermentation broth in the degradation of pesticide residues, ZL200410036024.9; Bai Hongjuan et al., Journal of Environmental Science, 2006, 26(11); Bai Hongjuan et al., Chinese Journal of Safety Science , 2007, 17(1)), but its research is still based on laboratory research, and it is a separate study of pesticides and metals, and there is no report on the application technology of photosynthetic bacteria fermentation broth bioremediation of carbamate pesticides and metal compound pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Prepare the culture medium with the following components:

[0027] Sodium acetate 1500mg CaCl 2 2H 2 O 80mg

[0028] MgSO 4 ·7H 2 O 150mg EDTA 20mg

[0029] Yeast Extract 1200mg K 2 HPO 4 1000mg

[0030] (NH 4 ) 2 SO 4 1500mgKH 2 PO 4 800mg

[0031] FeSO 4 ·7H 2 O 10mg trace element solution 3ml

[0032] Deionized water 1500ml

[0033] Wherein the composition of the trace element solution is: H 3 BO 3 240 mg; Na 2 MoO 4 2H 2 O 70mg; CuSO 4 7 mg; MnSO 4 4H 2 O 200mg; ZnSO 4 ·7H2 O 24mg; deionized water 1650ml.

[0034] The prepared medium has a pH value of 7.

[0035] Insert 1 / 20 portion of Rhodobacter sphaeroides into the sterilized culture medium, and culture it under 2500 lux light, room temperature and anaerobic conditions for 20 days to obtain photosynthetic bacterium Rhodobacter sphaericus fermentation broth.

[0036] In one acre of soil with carbofuran pollution concentration of 15mg / kg, cadmium pollution concentra...

Embodiment 2

[0038] The photosynthetic bacterium Rhodopseudomonas palustris fermented liquid was cultivated using the culture medium and culture method of Example 1.

[0039] In an acre of soil with aldicarb pollution concentration of 10mg / kg, lead pollution concentration of 500mg / kg soil, and pH=8.5 soil, the treated soil was flooded 2 days before spraying the bacteria agent, and 30kg of soil containing effective viable bacteria 5×10 8 The fermented liquid of Rhodopseudomonas palustris per ml mixed with 80 times of water was sprayed on the treated soil. After the spraying, the ground surface was plowed about 20cm by machine to fully mix the bacterial liquid with the soil. Sampling detection on the 15th day, the degradation rate of aldicarb was 94%, and the Pb in the soil 2+ Bioavailability is reduced by 70%.

Embodiment 3

[0041] The culture medium and culture method of Example 1 were used to cultivate the mixed bacteria fermentation broth of photosynthetic bacteria Rhodopseudomonas aeruginosa and Marine Rhodopseudomonas.

[0042] In an acre of soil with a concentration of 30mg / kg of chlorphenamine pollution, a soil of 600mg / kg of copper pollution, and pH=6.5, the treated soil was flooded 2 days before spraying the bacterial agent, and 15kg of soil containing effective viable bacteria was used. 8×10 9 The mixed bacterial fermentation liquid of Rhodopseudomonas viridans and Oceanic Rhodopseudomonas per ml was sprayed on the treated soil with 100 times of water. After the spraying was completed, the ground surface was plowed about 20cm by machine to make the bacteria liquid and the soil Mix well. On the 15th day, the sampling detection showed that the degradation rate of chlorphenamine was 93%, and Cu in the soil 2+ Bioavailability is reduced by 75%.

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PUM

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Abstract

The invention provides application of photosynthetic bacteria fermentation broth in pesticide and metal combined contamination soil. In the application, the photosynthetic bacteria fermentation broth with the number effective viable bacteria to be between 108 and 1,010 per millimeter is diluted to 10 to 150 times with water; the fermentation broth is uniformly sprayed to degrading bacteria treated soil in dosage of between 10 and 50kg per mu; the earth surface is turned over for about 20 cm after the spraying is completed to ensure that the broth and the soil are sufficiently mixed and the photosynthetic bacteria can degrade residues of carbamic acid ester pesticide in the soil in high efficiency; and simultaneously, the bioavailability of the metal can be reduced to repair the carbamic acid ester pesticide and metal combined contamination soil. The photosynthetic bacteria has wide raw material sources, low price and easily controlled growing condition, can use low molecular organic substances as photosynthetic electron receptor to perform photo heterotrophic growth under anaerobic illuminating condition, can use organic substances as breathing substrate to perform aerobic or heterotrophic growth under dark and aerobic conditions, can carry out the photosynthesis without releasing oxygen, and is suitable for large-scale industrial implementation.

Description

technical field [0001] The invention relates to a microbial application technology, in particular to the application of a photosynthetic bacteria fermentation liquid in the remediation of carbamate pesticides and metal compound polluted soil. Background technique [0002] Soil is the material basis for human survival and an important part of the ecological environment. In recent years, more and more attention has been paid to the pollution of soil pesticides and heavy metals, and bioremediation technology has also become a hot spot in the research of soil pollution remediation. There have been many literature reports on the microbial degradation of soil heavy metals and pesticides (Wang CL, et al.Appl Environ Microbiol, 2000, 66; Bang W, et al.Biotechnol Lett, 2000, 22; Natural Science Edition), 2003, 36(4); Zhang Deyong et al., Life Science Research, 2005, 9(3); Wu Jun et al., Journal of Environmental Science, 2004, 24(2); Jiang Jiandong et al., Bioengineering Journal, 200...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C12N1/20C12R1/01C12R1/38
Inventor 白红娟贾万利柴艳芳叶增民仪治本肖根林张肇铭
Owner ZHONGBEI UNIV
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