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Combined test reagent card for cytomegalovirus and rubella virus

A technology for cytomegalovirus and rubella virus, applied in the field of joint detection cards, can solve the problems of many factors affecting IgM antibody detection, prone to false positives, short maintenance time, etc., achieve high accuracy, broad application prospects, and reduce procedures Effect

Active Publication Date: 2010-06-16
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the laboratories use the single index of IgM antibody in early pregnancy serum as the index of cytomegalovirus and rubella virus infection in pregnant women, but there are problems only from the perspective of methodology, because there are many factors affecting the detection of IgM antibody, mainly false positives are prone to occur, and Antibody is the earliest to be produced, once infected, it is produced quickly, and it works against infection in the early stage of infection, but it lasts for a short time and disappears quickly

Method used

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  • Combined test reagent card for cytomegalovirus and rubella virus
  • Combined test reagent card for cytomegalovirus and rubella virus
  • Combined test reagent card for cytomegalovirus and rubella virus

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Example 1: Such as figure 1 with figure 2 As shown, a cytomegalovirus and rubella virus combined detection reagent test card includes a card cover 1, a card bottom 2 and a test strip 3. The card cover 1 is provided with a sample hole 11, an observation hole 12 and an installation marking hole 13 , The card bottom 2 is provided with a groove 21 in which the test strip 3 is placed. The test sample application area 31 on the test strip 3 corresponds to the sample injection hole 11 on the card cover 1; the gold label area 32 and the nitrocellulose area 33 on the test strip 3 correspond to the observation hole 12 on the card cover 1. correspond.

[0044] Figure and Figure 4 As shown, the test strip 3 is provided with a test sample area 31, a gold label area 32, a nitrocellulose membrane area 33, and a water absorption area 34 in sequence. The test sample area 31 is a glass fiber layer; the gold label area 32 is provided with a test strip 3 On the upper surface of the colloi...

Embodiment 2

[0071] Embodiment 2: A cytomegalovirus and rubella virus combined detection reagent test card, the structure of which is the same as that of embodiment 1.

[0072] A method for preparing a cytomegalovirus and rubella virus combined detection reagent detection card is assembled from the above-mentioned card cover 1, card bottom 2 and test strip 3, and the test strip 3 is sequentially provided with a detection sample area 31 and a gold label area 32. The nitrocellulose membrane area 33 and the water absorption area 34. The gold label area 32 is provided with a colloidal gold layer 321. The colloidal gold layer 321 consists of chloroauric acid solution, trisodium citrate solution, potassium carbonate solution and anti-human IgG monoclonal antibody The solution is prepared.

[0073] The preparation process of the colloidal gold layer 321 is as follows:

[0074] A. Take 100ml of 4% chloroauric acid aqueous solution, heat it to boiling, add 0.8ml 0.5% trisodium citrate solution, mix quick...

Embodiment 3

[0085] Embodiment 3: A cytomegalovirus and rubella virus combined detection reagent test card, the structure of which is the same as that of embodiment 1.

[0086] A method for preparing a cytomegalovirus and rubella virus combined detection reagent detection card is assembled from the above-mentioned card cover 1, card bottom 2 and test strip 3, and the test strip 3 is sequentially provided with a detection sample area 31 and a gold label area 32. The nitrocellulose membrane area 33 and the water absorption area 34. The gold label area 32 is provided with a colloidal gold layer 321. The colloidal gold layer 321 consists of chloroauric acid solution, trisodium citrate solution, potassium carbonate solution and anti-human IgG monoclonal antibody The solution is prepared.

[0087] The preparation process of the colloidal gold layer 321 is as follows:

[0088] A. Take 100ml of 2% chloroauric acid aqueous solution, heat to boiling, add 0.8ml of 0.8% trisodium citrate solution, mix quick...

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Abstract

The invention discloses a combined test reagent card for cytomegalovirus and rubella virus, which comprises a card cover, a card bottom and a test strip. The test strip is provided with a test sample region, a gold label region, a nitrocellulose membrane region and a water absorption region sequentially; and the nitrocellulose membrane region is provided with a quality-controlling line coated with goat anti mouse IgG, a testing line coated with cytomegalovirus antigen and a testing line coated with rubella virus antigen sequentially. The technical scheme adopted by the invention can test cytomegalovirus IgG antibody and rubella virus IgG antibody in the sample simultaneously and the total accordance rate of the testing result is higher, so the application prospect is wide. Compared with the detection of IgM antibody, the combined test reagent card for the cytomegalovirus and the rubella virus tests the IgG antibody; the IgG antibody can be carried for the whole life and is only associated with acute infection; and the accuracy is high.

Description

Technical field [0001] The invention belongs to the field of biological detection, and specifically relates to a combined detection card capable of simultaneously detecting cytomegalovirus (CMV) IgG antibody and rubella virus (RV) IgG antibody. Background technique [0002] Cytomegalovirus (Cytomegalovirus, CMV) is also known as cellular inclusion body virus. It is named because the infected cells are enlarged and have huge nuclear inclusion bodies. Cytomegalovirus infection is very widespread in the population. The adult infection rate in my country is more than 95%. It is usually recessive. Most infected people have no clinical symptoms, but under certain conditions, they can invade multiple organs and systems and cause serious diseases. The virus can invade the lungs, liver, kidneys, salivary glands, other glands of the breast, as well as polynucleated white blood cells and lymphocytes. It can be discharged from saliva, breast sweat, blood, urine, semen, and uterine secretions...

Claims

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Application Information

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IPC IPC(8): G01N33/571G01N33/532G01N33/577
Inventor 杨致亭来秀芬刘善利傅爱华范永熙
Owner 山东康华生物医疗科技股份有限公司
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