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Gene engineering application of rice auxin transport protein gene OsPIN2

A technology of genetic engineering and auxin, applied in the field of genetic engineering application, gene encoding auxin transport protein and its regulation in rice, can solve the unreported problems of improving nitrogen use efficiency and yield traits, etc.

Inactive Publication Date: 2012-05-16
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Excessive or suppressed expression of OsPIN1 through transgenic methods will lead to changes in rice root-shoot ratio (XuM, Zhu L, Shou H and Wu P. Plant Cell Physiol. A PIN1 family gene, OsPIN1, involved in auxin-dependentadventitious root emergence and tillering in rice.2005, 46(10): 1674-1681.), but did not report the improvement of nitrogen use efficiency and yield traits

Method used

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  • Gene engineering application of rice auxin transport protein gene OsPIN2
  • Gene engineering application of rice auxin transport protein gene OsPIN2
  • Gene engineering application of rice auxin transport protein gene OsPIN2

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Embodiment Construction

[0040] (1) Expression characteristics of OsPIN2 gene (Accession No. AK101191) in rice

[0041] 1) Extraction of total RNA. Rice Nipponbare seeds are sterilized by mass ratio 30% NaClO, accelerated germination, and when two leaves and one heart are cultivated, rice plants of the same size are selected, and the endosperm is removed and transplanted to 1 / 2 of the International Rice Institute with pH 5.5. In the IRRI nutrient solution, replace it with the IRRI complete nutrient solution from the International Rice Institute (Mao D R. The methods of plant nutrition research. Beijing: Beijing Agricultural University Press, 1994.) when the four leaves are in one heart. Freeze and store in liquid nitrogen, weigh about 0.1g sample, grind it with liquid nitrogen, grind it fully and add it to a 1.5ml centrifuge tube, quickly add 1ml Trizol reagent, add 0.2mL chloroform, absorb the supernatant after centrifugation, add 0.5mL isopropyl After centrifugation, discard the supernatant, add 70%...

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Abstract

The invention discloses gene engineering application of rice auxin transport protein gene OsPIN2, which belongs to the field of gene engineering. The accession numbers of the nucleotide sequence of the rice auxin transport protein gene OsPIN2 and the expression OsPIN2 protein amino acid sequence thereof are AK101191 in an NCBI website (www. ncbi. nlm. nih. Gov / ). The engineering application of the gene is firstly reported in the rice, and participates in the transport of rice auxin so as to increase the rice roots, tiller number, tiller angle, nitrogen utilization efficiency and finial output.

Description

technical field [0001] The invention discloses the genetic engineering application of the rice auxin transport protein gene OsPIN2, belongs to the technical field of genetic engineering, and specifically relates to the application of the gene encoding the auxin transport protein and its regulation in rice. Background technique [0002] Nitrogen is one of the important macronutrients for crops and participates in various metabolic processes of organisms. It is a component of many living substances in plants, such as: amino acids, proteins, nucleic acids, enzymes, chlorophyll, etc. Nitrogen accounts for 1.5-2% of plant body dry weight and 16% of total plant protein (Frink CR., Waggoner PE.and Ausubel JH.Nitrogen fertilizer: retrospect and prospect.Proc.Nati.Acad.Sci.USA.1999.96: 1175~1180.). At present, China's nitrogen fertilizer use accounts for 30% of the global nitrogen fertilizer use (Peng Shaobing, Huang Jianliang, Zhong Xuhua, Yang Jianchang, Wang Guanghuo, Zou Yingbi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H4/00A01H5/00
Inventor 范晓荣徐国华陈赢男张亚丽
Owner NANJING AGRICULTURAL UNIVERSITY
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