Cardiac reentry model chip and apparatus and method for evaluating drug using the cardiac reentry model chip

Abstract

It is intended to develop a chip by which cell potential and cell morphology can be accurately measured on a single cell basis and a heart model comprising a closed loop having myocardial cells and fibroblasts in an appropriately dispersed state can be constructed to evaluate the effect of a drug. An in vitro cardiac reentry model chip is fabricated by constructing a closed loop, wherein myocardial cells and fibroblasts are provided on a transparent electrode formed on a transparent substrate by using a constitution of enclosing a single cell in a specific spatial configuration, and transmitting the pulsating of an arbitrary myocardial cell or a specific myocardial cell in the closed loop to the both sides of the loop so as to electrically detect the pulsating conditions of the cell in the closed loop. The usefulness or toxicity of a drug to myocardial cells is evaluated by loading the drug on this cardiac reentry model chip and measuring the cell potentials of the individual cells.

Description

technical field [0001] The invention relates to a cardiac reentry model chip (Cardiac Reentry Model Chip) and a drug evaluation device and method using the cardiac reentry model chip. Background technique [0002] Bioassays are often used to observe cell state changes, cell responses to drugs, and the like. In conventional bioassays, cultured cells are generally used in many cases. Since measurement is performed using a plurality of cells in this system, the average value of the values ​​of the cell population is observed as a characteristic of one cell. [0003] However, in practice, cell cycle synchronization in a cell population is rare, and individual cells express proteins with different cycles. Therefore, when interpreting the results of responses to stimuli, there is always the problem of fluctuations. [0004] That is, since the response mechanisms of cells themselves have fluctuations in response that are generally present, only an average response can often be o...

AI Technical Summary

Problems solved by technology

If the number of cells is too large, as described in the above-mentioned prior art, there is a problem that the obtained information becomes average information, and the response to drugs, etc. cannot be obtained correctly.

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