Method for relieving stress response in manual operations of juvenile fish of sebastes schlegeli hilgendorf
A technology of stress response and manual operation, applied in application, fish farming, climate change adaptation, etc., can solve problems such as surface injury of fish, physiological dysfunction of aquatic animals, redness and swelling of wounds, etc.
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Embodiment 1
[0017] In August 2009, in the seed cultivation workshop of Yantai Baijia Aquatic Products Co., Ltd. located in Huangshanguan Town, Longkou City, Yantai, the water temperature was 25-26°C, salinity 29-30, pH 8.0-8.2, DO>6mg / L The seawater, with a 20L plastic storage box as the container, the volume of seawater is 10L, a small amount of air. The juveniles of Xu's flat scorpionfish have a total length of 59.6mm ± 6mm (average total weight 3.79g), and fast for one day.
[0018] Use MS-222 produced by Fluka to prepare MS-222 mother liquor: Dissolve MS-222 in distilled water according to the ratio of MS-222: distilled water 1g: 10mL, then the density of the mother liquor is 0.1g / mL. Calculated according to the concentration of 100mg / L, 1.0g of MS-222 should be dissolved in 10L of seawater, that is, add 10mL of MS-222 mother liquor.
[0019] Use a net to fish the juvenile scorpionfish into the MS-222 seawater solution. Within 3 minutes, the juvenile fish lie on the bottom of the tan...
Embodiment 2
[0021] In September 2009, in the seed cultivation workshop of Yantai Baijia Aquatic Products Co., Ltd. located in Huangshanguan Town, Longkou City, Yantai, seawater with a water temperature of 22°C, a salinity of 29-30, a pH of 8.0-8.2, and a DO>6mg / L was used , with a 200L PVC tank as a container, seawater volume of 100L, a small amount of air. The juveniles of Xu's flat scorpionfish have a total length of 95.0mm ± 7mm (average total weight 13.79g), and fast for one day.
[0022] Use MS-222 produced by Fluka to prepare MS-222 mother liquor: Dissolve MS-222 in distilled water according to the ratio of MS-222: distilled water 1g: 10mL, then the density of the mother liquor is 0.1g / mL. Calculated according to the concentration of 110mg / L, 11.0g of MS-222 should be dissolved in 100L of seawater, that is, 110mL of MS-222 mother liquor should be added.
[0023] Use a net to fish the juvenile Scorpene scorpionfish into the MS-222 seawater solution. Within 3 minutes, the juvenile fi...
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