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Rapid breeding method for pinellia tuber tissue culture

A tissue culture and Pinellia chinensis technology, which is applied in the field of biotechnology engineering, can solve the problems of lack of resources and difficulty in breeding Pinellia chinensis, and achieve the effects of promoting healthy development, increasing yield, and expanding planting areas.

Inactive Publication Date: 2010-06-09
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Use the method of the present invention to carry out large-scale rapid propagation of Pinellia pinellia to solve the problem of the difficulty in breeding Pinellia pinellia in the natural state and the increasingly scarce resources of Pinellia pinellia, realize large-scale artificial planting of Pinellia pinellia, and increase the reserves of Pinellia pinellia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 (with dry pinellia leaf as explant):

[0032] First use the tubers of Pinellia chinensis to make callus differentiation and culture to obtain sterile seedlings, and then use the leaves as explants to induce callus.

[0033] (1) The method for germination of aseptic seedlings of Pinellia pinellia is as follows: soak the tubers of Pinellia pinellia with 75% alcohol for 45 seconds, then sterilize them with 0.1% mercuric chloride for 15 minutes, rinse them with sterile water for 5 times, and cut them into Small pieces of 5mm in size, inoculated on MS+KT 2.0mg / L+2,4-D 2.0mg / L medium to obtain callus, then moved to MS+KT 3.0mg / L+2,4-D 1.0 mg / L medium, and the differentiated cluster buds were cut into single shoots (containing basal callus pieces) and allowed to continue to grow on MS medium. After culturing for 30 days at a temperature of 25° C. under a light of 2000 Lx and a light of 12 h / d, the aseptic seedlings of Pinellia can be obtained.

[0034] (2) Inser...

Embodiment 2

[0039] Embodiment 2 (with the dry Pinellia petiole as explant):

[0040] The callus was differentiated and cultivated to obtain aseptic seedlings firstly, and then its petiole was used as explant to induce callus.

[0041] (1) The method for germination of aseptic seedlings of Pinellia pinellia is as follows: soak the tubers of Pinellia pinellia with 75% alcohol for 45 seconds, then sterilize them with 0.1% mercuric chloride for 15 minutes, rinse them with sterile water for 5 times, and cut them into Small pieces of 5mm in size, inoculated on MS+KT 2.0mg / L+2,4-D 2.0mg / L medium to obtain callus, then moved to MS+KT 3.0mg / L+2,4-D 1.0 mg / L for differentiation, and the differentiated cluster buds were cut into single buds (including basal callus pieces) to continue to grow on MS medium. After cultivating for 30 days at a temperature of 25° C. under a light of 2000 LX, the aseptic seedlings of Pinellia rhizome can be obtained.

[0042] (2) directly insert the petiole of the asept...

Embodiment 3

[0047] Embodiment 3 (with the dry pinellia callus piece as explant):

[0048] The callus was differentiated and cultivated to obtain aseptic seedlings firstly, and then the callus was used as explant to continue to induce callus.

[0049] (1) The method for germination of aseptic seedlings of Pinellia pinellia is as follows: soak the tubers of Pinellia pinellia with 75% alcohol for 45 seconds, then sterilize them with 0.1% mercuric chloride for 15 minutes, rinse them with sterile water for 5 times, and cut them into Small pieces of 5mm in size, inoculated on MS+KT 2.0mg / L+2,4-D 2.0mg / L medium to obtain callus, then moved to MS+KT 3.0mg / L+2,4-D 1.0 mg / L for differentiation, and the differentiated cluster buds were cut into single buds (including basal callus pieces) to continue to grow on MS medium. After cultivating for 30 days at a temperature of 25° C. under a light of 2000 Lx, the aseptic seedlings of Pinellia rhizome can be obtained.

[0050] (2) direct access to the med...

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PUM

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Abstract

The invention relates to a rapid breeding method for pinellia tuber tissue culture, comprising the following steps: using a tissue culture technology to induce sterile pinellia tuber to generate callus and generate sterile seeding by differentiation; taking leaves, petioles and the callus pieces of the sterile seeding as explants; and inducing by the callus, differentiation culturing, enrichment culturing, radication culturing, and breeding the pinellia tuber seeding massively. The invention has the advantages of rich sources of explants, larger volume, high inoculation survival rate, simple and rapid operation, high breeding coefficient and quick speed and is suitable for industrialized seedling.

Description

technical field [0001] The invention belongs to the field of biotechnology engineering, and in particular relates to a rapid propagation technique of Pinellia chinensis tissue culture. Background technique [0002] Pinellia ternate (Thunb.) Breit. is a plant of the genus Pinellia in the family Araceae, and its dried tubers are used as medicine. , mostly used to treat phlegm, asthma, cough, nausea, vomiting, dizziness, etc. In recent years, pinellia protein has been found to have anti-early pregnancy and anti-tumor effects. At present, the annual domestic consumption has reached 5,000 tons, and it is one of the traditional Chinese medicinal materials that has been in short supply for many years. Almost all medicinal pinellia comes from the wild. Although my country has abundant wild pinellia germplasm resources, due to the large demand for medicinal and export of Pinellia pinellia, the wild resources are increasingly depleted and difficult to meet the requirements, and the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李昆志王艺霖屈文国陈丽梅
Owner KUNMING UNIV OF SCI & TECH
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