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Method for preparing broad spectrum antihumanglobulin cards

An anti-human globulin, broad-spectrum technology, applied in the field of transfusion medicine, can solve the problems of reduced sensitivity, high price, large gel gaps, etc., and achieve the effect of strong buffer capacity

Active Publication Date: 2010-06-02
JIANGYIN LIBO MEDICINE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, imported micro-column gel antiglobulin cards have been used in China to screen and identify irregular antibodies, detect sensitized red blood cells, etc. However, due to its high price and expensive equipment, its popularization in China has been seriously affected.
In addition, there is also the production of micro-column gel protein cards in China, but the gel used at home and abroad is dextran gel. The particle size of this gel is more than 70 nanometers, the particles are larger, and the gaps between the gels are larger. , which reduces the sensitivity of the detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1, preparation of gel suspension medium

[0041] Described gel suspending medium formula is as follows:

[0042] Methylparaben (5.5-6.5)×10 -4 g / ml

[0043] Propylparaben (1.0-1.5)×10 -4 g / ml

[0044] Glycine (1.6-1.9)×10 -2 g / ml

[0045] Sodium chloride (1.7-1.8)×10 -3 g / ml

[0046] Potassium dihydrogen phosphate (2.1-2.4)×10 -4 g / ml

[0047] Disodium hydrogen phosphate (4.6-4.8)×10 -4 g / ml

[0048] Bovine serum albumin ≤2%,

[0049] Dissolve the above reagents in distilled water and adjust the pH value to 6.6-6.8.

[0050] Step 2. Preparation of the gel

[0051] Polyacrylamide dextran gel is selected, and the particle size is 30-60 nanometers. Soak in the gel suspension medium prepared in step 1, then wash with the gel suspension medium for 3-5 times, remove the broken fragments of the gel and the aggregated gel particles, and collect the applicable gelatin powder with uniform particle size and complete spherical shape. gel.

[0052] Step 3. Antibod...

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Abstract

The invention relates to a method for preparing broad spectrum antihumanglobulin cards. Six micro-column gel tubes are arranged on the cards, and the six micro-column gel tubes are gel tubes containing an antibody for resisting IgG+C3d. The method comprises the following processes of preparation of a gel suspension medium, preparation of a gel, selection of the antibody, and preparation and sub-package of the gel. The gel suspension medium comprises the reagents of (5.5-6.5)*10-4g / ml methyl- p-hydroxybenzoate, (1.0-1.5)*10-4g / ml propyl p-hydroxybenzoate, (1.6-1.9)*10-2g / ml glycine, (1.7-1.9)*10-3g / ml sodium chloride, (2.1-2.4)*10-4g / ml monopotassium phosphate, (4.6-4.8)*10-4g / ml disodium hydrogen phosphate and less than or equal to 2 percent of bovine serum albumin. The reagents are dissolved by using distilled water; and the pH value is adjusted into 6.6-6.8. The broad spectrum antihumanglobulin cards prepared by the method have the advantages of high sensitivity, favorable specificity and stable quality.

Description

(1) Technical field [0001] The invention relates to a microcolumn gel blood type card used for blood type cross-matching, irregular antibody screening, and irregular antibody identification and its application. More specifically, the present invention relates to the composition, preparation method and application of a broad-spectrum anti-human globulin card, and relates to the field of blood transfusion medicine. (2) Background technology [0002] Micro-column gel antiglobulin card is a new immunoassay method that has gradually emerged recently. It is an anti-human globulin test performed on red blood cells with incomplete antibodies and corresponding antigens in a gel medium containing anti-human globulin. The method is the product of the combination of gel column and anti-human globulin test method. In 1984, Dr. Yves Lapierre in France first invented this method. Compared with the traditional test tube method of antiglobulin test, it omits the cumbersome washing procedure ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/80
Inventor 陈玉平钱国强朱庆平
Owner JIANGYIN LIBO MEDICINE BIOTECH
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