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Method for preparing butanedioic acid through fermentation

A technology of succinic acid and anaerobic fermentation, applied in the field of bioengineering, can solve the problems of increasing the difficulty of separation and extraction of downstream products, unfavorable industrial production of succinic acid biological method, large consumption, etc. Technical effect, effect of reducing difficulty

Inactive Publication Date: 2012-09-12
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

US5573931 reported that MgCO 3 It is a better neutralizer and can show better regulation ability, such as adding MgCO to the fermentation medium 3 solid, which can maintain the pH value of the fermentation system at an appropriate level, so that the bacteria show high metabolic activity during the fermentation process; however, MgCO 3 The cost is high, the consumption is large, and it will further increase the difficulty of separation and extraction of downstream products, which is actually not conducive to the industrial production of succinic acid biological method

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  • Method for preparing butanedioic acid through fermentation
  • Method for preparing butanedioic acid through fermentation
  • Method for preparing butanedioic acid through fermentation

Examples

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Embodiment 1

[0031] The method for preparing succinic acid described in the present invention can use any anaerobic fermentation microbial strain producing succinic acid in the prior art. The succinic acid-producing microbial strain used in this example is: Actinobacillus succinogenes NJ113 (Actinobacillus succinogenes NJ113). This bacterium has applied for a patent and obtained authorization, and the patent authorization announcement number is CN100537744C.

[0032] The fermentation medium of the present invention can use any fermentation medium for producing succinic acid by anaerobic fermentation in the prior art. The medium used in this example is: every liter of volume medium contains 50-100 g of glucose (sterilized separately), 15 g of yeast powder, 15 mL of corn steep liquor, 1.36 g of anhydrous sodium acetate, 1 g of NaCl, K 2 HPO 4 3g, MgCl 2 0.2g, CaCl 2 0.2g.

[0033] The method for preparing succinic acid by fermenting microbial strains of the present invention can be any...

Embodiment 2

[0043] The present embodiment adopts the same fermentation conditions and methods as in Example 1, only changing the method of adding alkaline neutralizing agent separately in the step of producing succinic acid as follows:

[0044] Add Ca(OH) separately 2 and CaCO 3 As an alkaline neutralizing agent to adjust the pH value.

[0045] Ca(OH) was used separately in the fermenter 2 , CaCO 3 Adjust the pH to ferment, the results are shown in figure 2 shown. Using Ca(OH) 2 Regulated, the thalline hardly grows, and the mass concentration of succinic acid is less than 5g / L ( figure 2 -a). CaCO 3 Solids adjust the pH of fermentation, and the growth ratio of bacteria is Ca(OH) 2 Slightly better when conditioning fermentation, but still noticeably inhibited. CaCO 3 The dissolving power of the bacterium is relatively poor, and the pH cannot be maintained at a level suitable for the growth of the thallus. During the fermentation process, the pH constantly decreases, and to the...

Embodiment 3

[0047] The present embodiment adopts the same fermentation conditions and methods as in Example 1, only changing the method of adding alkaline neutralizing agent separately in the step of producing succinic acid as follows:

[0048] Add NaOH, Na 2 CO 3 , NaHCO 3 As an alkaline neutralizing agent to adjust the pH value.

[0049] Feed NaOH, Na 2 CO 3 , NaHCO 3 The way to adjust the pH, the fermentation results are as follows image 3 shown. With Na + Continuously accumulating in the fermentation broth, the bacteria flocculation phenomenon is serious in the middle and late stages of fermentation, and the bacteria OD 660 There was an obvious downward trend, and the rate of sugar consumption gradually slowed down. NaOH and Na 2 CO 3 , NaHCO 3 Compared to the end of fermentation, the residual amount of glucose was more, and the mass concentration of succinic acid was 43.5g / L ( image 3 -a). Na 2 CO 3 , NaHCO 3 able to provide additional CO 3 2- 、HCO 3 - , which ...

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Abstract

The invention relates to a method for preparing butanedioic acid through fermentation. A fermentation system is kept to have a proper pH mainly by adding two types of mixed alkaline compound s instead of a single alkaline compound in the process of producing butanedioic acid through microbial fermentation, and the proportion of mixed alkali is regulated so as to ensure that the butanedioic acid produced is at a high level. The method not only can utilize the cheap alkaline compound s, but also can basically reach the level of butanedioic acid production when basic magnesium carbonate is utilized as the only alkaline compound to regulate pH. Therefore, by utilizing a mixed-alkali pH-regulating strategy to optimize a production process, the method has the advantages of reducing production cost of butanedioic acid biological preparation, reducing the difficulty of separating and extracting downstream products, increasing economic benefit and helping to the industrial preparation of butanedioic acid biological method.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for preparing succinic acid by using mixed alkali to adjust pH and ferment. Background technique [0002] Succinic acid, also known as succinic acid, is a dicarboxylic acid. As an important C4 platform compound, it is widely used in food, medicine, fragrance, detergent, fine chemical products, surfactant biodegradable materials, etc. The traditional production method of succinic acid is to refine and synthesize petroleum by chemical method. With the depletion of petroleum resources, the production of succinic acid by microbial fermentation is environmentally friendly, can utilize waste biomass resources, and can fix the greenhouse gas CO. 2 Such advantages have become a hot topic of research, and are being carried out in depth in the United States, Japan and other countries. [0003] CO 2 And pH value are the key factors affecting the growth of microbial cells an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/46C12R1/01
Inventor 姜岷李建陈可泉杨卓娜郑晓宇韦萍欧阳平凯
Owner NANJING TECH UNIV
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