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Phaffia rhodozyma culture method for improving content of astaxanthin in phaffia rhodozyma cells

A technology of Phaffia and astaxanthin, applied in the field of microbial biology, can solve the problems of low astaxanthin yield and unsuitability for industrialization

Inactive Publication Date: 2010-05-12
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The inventors have found that the yield of astaxanthin obtained by Phaffia yeast culture is not high at present, which does not meet the requirements of industrialization, and there is an urgent need to study methods for improving the content of Phaffia astaxanthin. Experiment and analysis research, finally finished the present invention

Method used

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Embodiment 1

[0069] Embodiment 1: the cultivation of Phaffia wild strain JCM9042 of the present invention

[0070] The PDA solid slant medium is prepared as follows: 200g of potatoes are washed, peeled and chopped, boiled with 1000ml of water for half an hour, filtered with gauze, and 20g of glucose is added to the filtrate, and after mixing, the liquid volume is supplemented with deionized water to 1000ml. Sterilize at a temperature of 115° C. for 15 minutes to obtain a PDA liquid medium, add 15 g of agar to the PDA liquid medium, and obtain a PDA solid medium after the agar is dissolved and solidified.

[0071] Preparation of seed medium: Dissolve 36-44g glucose, 4.0g yeast powder, 2.4g urea, 2.0g potassium dihydrogen phosphate, 0.5g magnesium sulfate heptahydrate in distilled water and set the volume to 1000ml, adjust the pH to 6.0.

[0072] Prepare fermentation medium: dissolve 110g glucose, 4.0g yeast powder, 0.6g urea, 2.0g potassium dihydrogen phosphate, 0.5g magnesium sulfate hepta...

Embodiment 2

[0083] Embodiment 2: the cultivation of Phaffia wild strain JCM9042 of the present invention

[0084] The PDA solid slant medium is prepared as follows: 200g of potatoes are washed, peeled and chopped, boiled with 1000ml of water for half an hour, filtered with gauze, and 20g of glucose is added to the filtrate, and after mixing, the liquid volume is supplemented with deionized water to 1000ml. Sterilize at a temperature of 115° C. for 15 minutes to obtain a PDA liquid medium, add 15 g of agar to the PDA liquid medium, and obtain a PDA solid medium after the agar is dissolved and solidified.

[0085] Preparation of seed medium: 40 g of glucose, 4.0 g of yeast powder, 2.4 g of urea, 2.0 g of potassium dihydrogen phosphate, and 0.5 g of magnesium sulfate heptahydrate were dissolved in distilled water and the volume was adjusted to 1000 ml, and the pH was adjusted to 6.0.

[0086] Preparation of fermentation medium: Dissolve 110g of glucose, 4.0g of yeast powder, 0.6g of urea, 2....

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Abstract

The invention relates to a phaffia rhodozyma culture method for improving the content of astaxanthin in phaffia rhodozyma, which comprises the steps of the preparation of solid seeds of a phaffia rhodozyma wild strain JCM9042, first-grade seed culture, second-grade seed culture, fermentation culture and the determination of the content of the astaxanthin in a fermentation broth. The culture method uses an ergosterol synthesized inhibitor, and can obviously promote the accumulation of the astaxanthin in the phaffia rhodozyma; and compared with a culture method which does not use the ergosterol synthesized inhibitor, the culture method can improve the content of the astaxanthin high up to more than 3 to 8 times. The method is simple and easy, has low costs of the ergosterol synthesized inhibitor and a culture medium, effectively reduces the production cost of the astaxanthin, and is favorable for industrialized culture.

Description

【Technical field】 [0001] The invention belongs to the field of microbial biotechnology. More specifically, the present invention relates to a method for cultivating Phaffia yeast to increase the content of astaxanthin in Phaffia yeast cells. 【Background technique】 [0002] Astaxanthin is an orange-red carotenoid that widely exists in nature and has strong antioxidant activity, which is 500 times that of vitamin E. Animal experiments have shown that astaxanthin has various physiological effects such as inhibiting tumor occurrence and enhancing immune function; it can prevent and treat neurological diseases, such as Alzheimer's disease and Parkinson's disease, and can be used as a therapeutic agent for anti-aging and preventing arteriosclerosis and other diseases. Drugs or health care products; can be used to prevent and treat eye diseases, especially to significantly reduce the incidence of diabetic eye diseases. [0003] The US Food and Drug Administration (FDA) and the Eu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
Inventor 李颖苗莉莉迟爽关国华王珍芳
Owner CHINA AGRI UNIV
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