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Rhamnose lactobacillus M8, rhamnose lactobacillus SLP and preparation method thereof

A technology of Lactobacillus rhamnosus and Lactobacillus, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of restricting the application of lactic acid bacteria, the function and mechanism of S-layer protein are not thorough, and achieve protection Effects on human health, improvement of human health, and extension of colonization time

Inactive Publication Date: 2010-03-17
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

However, there are not many known lactic acid bacteria containing S-layer proteins, and there are no more than 20 species included in GENBANK, and the research on the function and mechanism of S-layer proteins produced by these lactic acid bacteria is also very incomplete.
Due to the different adhesion ability of these lactic acid bacteria, the application of lactic acid bacteria in functional foods is seriously restricted.

Method used

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  • Rhamnose lactobacillus M8, rhamnose lactobacillus SLP and preparation method thereof

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Embodiment approach

[0022] A preferred embodiment of the present invention is as follows.

[0023] Step 1. Prepare the following materials and equipment:

[0024] Step 1.1 Prepare culture medium

[0025] Step 1.1.1 Prepare fresh MRS agar plates and MRS liquid medium, which are mainly used for the isolation of single colonies, activation and dilution of strains.

[0026] Step 1.1.2 Prepare the medium after transformation, its components include (mass percentage): 0.5% to 1.5% of collagen polypeptide, 0.5% of yeast extract, 0.5% of glucose, KH 2 PO 4 0.3%, Tween 800.5%, tomato juice 5%, sodium acetate 0.5% and sodium citrate 0.2%; pH 6.0.

[0027] Step 1.2 prepare buffer: 0.5mol / L sucrose, 7mmol / L KH 2 PO 4 and K 2 HPO 4 , 1mmol / L MgCl 2 , pH7.4.

[0028] Step 1.3 Prepare electroporation equipment: high-voltage pulse electroporation instrument.

[0029] Step 1.4 Obtain SLP protein gene

[0030] Step 1.4.1 Design primers: Design primers using Lactobacillus helveticus (ATCC 15009) surface pr...

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Abstract

The invention discloses a strain of rhamnose lactobacillus M8 and a strain of rhamnose lactobacillus SLP prepared from the same. The preparation method of the rhamnose lactobacillus SLP comprises thefollowing steps: taking the rhamnose lactobacillus M8 as a host, guiding lactobacillus surface protein genes into the host by an electrotransformation method, and incubating to successively express the lactobacillus surface protein genes in the host. The adhesive capability of the rhamnose lactobacillus SLP is greatly strengthened in the intestinal canal of a human body so as to efficiently strengthen the positive effect of the rhamnose lactobacillus in the alimentary canal of the human body.

Description

technical field [0001] The invention relates to a strain of Lactobacillus rhamnosus, and also relates to an engineering bacterium of Lactobacillus rhamnosus and a preparation method thereof. Background technique [0002] At present, there have been some studies and reports abroad on the use of surface protein (ie, S-layer protein) to colonize the human intestinal tract, and some have been applied to clinical treatment, such as severe diarrhea in infants caused by Helicobacter pylori infection, etc. The effect is very significant. However, the research on the colonization and cultivation of probiotics in my country has been progressing slowly, and there has been only related research on the colonization ability of pathogenic microorganisms in medicine. In recent years, the application of functional probiotics in food has been affirmed by domestic scholars and the general public. Functional foods in this field are emerging in an endless stream. How to improve the positive eff...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/21C12N15/74C12R1/225
Inventor 李宗军王远亮
Owner HUNAN AGRICULTURAL UNIV
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