SSR markers of powdery mildew resistance gene in wheat Fu Zhuang 30 and method for acquiring same

A powdery mildew resistance gene and marker technology, applied in the field of crop genetics and breeding, can solve the problem of few SSR markers of Pm5e gene, achieve strong polymorphism, improve breeding efficiency, and shorten the breeding period

Inactive Publication Date: 2010-03-03
李祥
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Problems solved by technology

At present, there are few reports on the reported SSR markers of the Pm5e gene
However, molecular markers closely linked to it have not been reported

Method used

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  • SSR markers of powdery mildew resistance gene in wheat Fu Zhuang 30 and method for acquiring same
  • SSR markers of powdery mildew resistance gene in wheat Fu Zhuang 30 and method for acquiring same
  • SSR markers of powdery mildew resistance gene in wheat Fu Zhuang 30 and method for acquiring same

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Embodiment Construction

[0035] The winter wheat variety Rejuvenation 30 used in this experiment was from Jinghui Farm, Shaanxi Province, and was bred from Jingyang 30 line. It carries a recessive gene against powdery mildew, which is highly resistant or immune to the popular powdery mildew races in my country. . In this study, rejuvenation 30 and the susceptible variety Chancellor were used as materials, and F 2 The powdery mildew resistance gene of Rejuvenation 30 was analyzed by SSR markers by segregation group analysis (BSA).

[0036] There are few SSR markers of Pm5e obtained so far, and molecular markers closely linked to it have not been reported.

[0037] In this study, three SSR markers linked to Pm5e gene were screened out, two of which were closely linked markers, which could be effectively used for marker-assisted selection of Pm5e gene. Using this marker as a guidepost, the fine positioning of the Pm5e gene can be carried out or the Pm5e gene can be approached by chromosome walking metho...

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Abstract

The invention discloses SSR markers of powdery mildew resistance gene in wheat Fu Zhuang 30 and a method for acquiring the same, which belong to the field of crop genetic breeding science. In the research, three SSR markers linked with the powdery mildew resistance gene Pm5e in Fu Zhuang 30 are screened out, wherein two of the three SSR markers are closely linked markers and can be effectively used in the marker auxiliary selection of the powdery mildew resistance gene Pm5e in Fu Zhuang 30. With the markers as road signs, the fine positioning of the Pm5e gene or the approach to the Pm5e gene by a genome walking method can be performed. Thus, the markers lay a foundation for the clone of the Pm5e gene.

Description

1. Technical field [0001] The invention provides the SSR marker of the wheat powdery mildew resistance gene Rejuvenation 30 and its obtaining method, which provides a molecular marker closely linked with the wheat powdery mildew resistance gene Rejuvenation 30, and belongs to the field of crop genetics and breeding. It can be effectively used for tracking detection and marker-assisted breeding of Rejuvenation 30. 2. Background technology [0002] Wheat powdery mildew, caused by the obligate parasitic fungus Erysipha tritici, is a worldwide disease that endangers wheat production. Wheat powdery mildew has the characteristics of many small species, rapid variation, and wide adaptability. When resistance genes are used in wheat production, powdery mildew populations often undergo co-evolution due to the selection of host resistance genes to overcome the resistance genes, resulting in Disease pandemic (Acta Phytopathology, 1995, 25:116). In recent years, powdery mildew has fre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/29
Inventor 李祥
Owner 李祥
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