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Selective coloration culture medium of clostridium perfringens

A technology for Clostridium perfringens and chromogenic medium, which is applied in the directions of microorganism-based methods, microorganism determination/inspection, microorganisms, etc., can solve the problems of long cultivation time, lack of diagnostic specificity, etc., and achieve shortened cultivation time Effect

Inactive Publication Date: 2010-03-03
CHENGDU RICH SCI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the above status quo, the present invention provides a selective chromogenic medium for Clostridium perfringens, which solves the problems of long culture time and lack of diagnostic specificity in the above-mentioned traditional culture identification methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Provides a medium for the rapid cultivation and identification of Clostridium perfringens. The main ingredients are: 12.0g of caseinase hydrolyzate, 12.0g of yeast extract powder, 0.20g of sodium sulfite, 0.015g of polymyxin B, 0.05g of sulfadiazine, 0.80g of ferric citrate, 4 units of pancreatin and 18.0g of agar powder. Accurately weigh the above ingredients, fully dissolve them with double distilled water, and set the volume to 1000ml with a volumetric flask. 3 ) under the conditions of high pressure steam sterilization, subpackage in sterile plates for future use, and the final pH (25°C) value is 6.8. Cultivate with this medium at 35°C for 8 hours, Clostridium perfringens grows well, the colonies appear black, and other sulfite-reducing Gram-positive and negative bacteria do not grow, so as to realize rapid cultivation and identification of gas production Clostridium perfringens.

Embodiment 2

[0020] Provides a medium for the rapid cultivation and identification of Clostridium perfringens. The main ingredients are: 20.0g of caseinase hydrolyzate, 6.0g of yeast extract powder, 0.80g of sodium sulfite, 0.005g of polymyxin B, 0.20g of sulfadiazine, 0.20g of ferric citrate, 12 units of pancreatin and 10.0g of agar powder. Accurately weigh the above ingredients, fully dissolve them with double distilled water, and set the volume to 1000ml with a volumetric flask. 3 ) under the conditions of high pressure steam sterilization, subpackage in sterile plates for later use, and the final pH (25°C) value is 7.2. When cultured at 37°C for 12 hours with this medium, Clostridium perfringens grows well, the colonies appear black, and other sulfite-reducing Gram-positive and negative bacteria do not grow, thus realizing rapid culture and identification of gas production Clostridium perfringens.

Embodiment 3

[0022] Provides a medium for the rapid cultivation and identification of Clostridium perfringens. The main components are: 14.0g of caseinase hydrolyzate, 10.0g of yeast extract powder, 0.40g of sodium sulfite, 0.01g of polymyxin B, 0.10g of sulfadiazine, 0.60g of ferric citrate, 6 units of pancreatin and 16.0g of agar powder. Accurately weigh the above ingredients, fully dissolve them with double distilled water, and set the volume to 1000ml with a volumetric flask. 3 ) under the conditions of high pressure steam sterilization, subpackage in sterile plates for future use, and the final pH (25°C) value is 7.0. When cultured at 36°C for 10 hours with this medium, Clostridium perfringens grows well, the colonies appear black, and other sulfite-reducing Gram-positive and negative bacteria do not grow, so as to realize rapid culture and identification of gas production Clostridium perfringens.

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Abstract

The invention relates to a selective coloration culture medium for fast culturing and identifying clostridium perfringens. The selective coloration culture medium is characterized in that the preparation of 1000ml of the culture medium requires 12.0g-20.0g of casease hydrolysate, 6.0g-12.0g of yeast extract powder, 0.20g-0.80g of sodium sulfite, 0.005g-0.015g of polymyxin B, 0.05g-0.20g of sulfadiazine, 0.20g-0.80g of ferric citrate, 4-12 units of Y.S.N. and 10.0g-18.0g of agar powder and also requires the final addition of distilled water till the volume of 1000ml is achieved. The selective coloration culture medium has the advantages that the culturing time is obviously reduced when being compared with a conventional method and other bacteria with the same culturing characteristics are inhibited and do not grow so that the effect of fast clostridium perfringens culturing and identification can be realized.

Description

Technical field: [0001] The invention relates to a selective chromogenic medium for rapid cultivation and identification of Clostridium perfringens. Background technique: [0002] Clostridium perfringens is widely distributed in nature and in the intestinal tract of humans and animals, and can cause various diseases in humans and animals, the most common clinical ones are gas gangrene and food poisoning caused by Clostridium perfringens . Gas gangrene is a severe acute infection caused by pathogenic bacteria invading the wound. It is characterized by tissue necrosis, edema, flatulence, and systemic poisoning. The disease is often a mixed infection involving two or more bacteria, 60%-80% of which are caused by Clostridium perfringens. Therefore, it is extremely important for the diagnosis and treatment of diseases to isolate and culture this bacterium from specimens early and quickly. Clostridium perfringens is a large gram-positive bacillus, anaerobic but not demanding. I...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/145
Inventor 王军
Owner CHENGDU RICH SCI IND
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