Method for tissue culture of liquidambar styraciflua

Abstract

The invention relates to a tissue culture method for plants, and in particular relates to a method for tissue culture of liquidambar styraciflua. The method comprises the following steps: selecting effective stems with axillary buds of liquidambar styraciflua as an explant, performing pretreatment such as sterilization on the effective stems firstly, subsequently inoculating the explant onto an axillary bud development starting culture medium for culture on an ultra-clean working table so as to obtain sterile seedlings; inoculating the sterile seedlings into a bud proliferation and elongation culture medium for culture, selecting growing points of the proliferated buds, sequentially transferring into a callus induction culture medium, a callus proliferation culture medium and a root induction culture medium for culture according to culture progress so as to obtain non-poisonous plants, and transplanting and planting the non-poisonous plants. According to the method, the unique explant and the formulae of the culture mediums are adopted, the success rate and the plant quality in tissue culture of liquidambar styraciflua are greatly improved, and strong guarantee for industrial development of liquidambar styraciflua is provided, and the method is rapid in culture and economical.

Description

technical field [0001] The invention relates to a tissue culture method of plants, in particular to a tissue culture method of ornamental trees, in particular to a tissue culture method of American red maple. Background technique [0002] American red maple (Acerrubrum), also known as red maple and North American red maple, belongs to the genus Aceraceae. The height of the tree is 12-18 meters. It has beautiful tree posture, luxuriant branches and leaves, delicate leaf shape, beautiful leaf color, and the earliest color change in autumn. The main color is bright red, the secondary color is orange, and the color is as bright as flowers. It is mainly used for forest garden viewing and garden greening. It is also a precious family potted tree species. At present, American red maple generally adopts three propagation methods: seed propagation, grafting propagation and cutting propagation, but the propagation speed of these three methods is relatively slow. Tissue culture rapid ...

AI Technical Summary

Problems solved by technology

At present, American red maple generally adopts three propagation methods: seed propagation, grafting propagation and cutting propagation, but the propagation speed of these three methods is relatively slow. Tissue culture rapid propagation is an effective propagation method, which not only improves the propagation of red maple speed, and at the same time, it also provides a strong guarantee for the quality of seedlings, providing sufficient seedlings for landscaping