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Method for in-situ dynamic observation and measurement of root system of plant

A technology for dynamic observation of plant roots, applied in the fields of crop cultivation, plant physiology, root biology, and plant nutrition, can solve problems that have not been seen in technical reports, and achieve the effect of simple and effective methods and shortened time

Inactive Publication Date: 2011-06-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, most studies on root architecture and nutrient efficiency are qualitative studies, and there are few reports on quantitative research on root architecture and nutrient efficiency.
In addition, most of the existing research is the study of root architecture data and nutrient efficiency at a certain moment, and there are no technical reports on the dynamic in-situ root architecture data and nutrient efficiency.

Method used

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  • Method for in-situ dynamic observation and measurement of root system of plant
  • Method for in-situ dynamic observation and measurement of root system of plant
  • Method for in-situ dynamic observation and measurement of root system of plant

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The in-situ observation and determination of the morphological configuration of the soybean root system of embodiment 1

[0039] (1) Design transparent cultivation containers and growth media;

[0040] as attached figure 1 As shown, a transparent cylindrical plexiglass container 1 is adopted, with a height of 20 cm and a diameter of 12 cm. An openable cover 2 is provided, and a through hole 3 with a diameter of 20 mm is arranged in the middle of the cover;

[0041] The transparent medium is modified 1 / 2 Hoagland nutrient solution + 0.15% Phytagel TM (W / V) + different phosphorus treatments including no phosphorus treatment or 1.0mM KH 2 PO4 For processing, refer to the conventional techniques of those skilled in the art, adjust the pH value to 5.8, and use hydrochloric acid or sodium hydroxide and other reagents to adjust according to conventional techniques.

[0042] The composition of 1 / 2 Hoagland nutrient solution is (μM): KNO 3 2.5×10 3 , MgSO 4 ·7H 2 O1×10 ...

Embodiment 2

[0060] The in-situ observation and determination of the morphological configuration of the rice root system of embodiment 2

[0061] (1) Design transparent cultivation containers and growth media;

[0062] Adopt a transparent cylindrical plexiglass container with a height of 20cm and a diameter of 12cm, with an openable cover, and a through hole with a diameter of 20mm in the middle of the cover;

[0063] The rice medium is: Yoshida improved rice nutrient solution in 1976 + 0.15% Phytagel TM (W / V) + different phosphorus treatments including no phosphorus treatment or 1.0mM KH 2 PO 4 For the treatment, refer to the conventional techniques of those skilled in the art, adjust the pH value to 5.7, and use hydrochloric acid or sodium hydroxide and other reagents to adjust according to conventional techniques.

[0064] The composition of rice nutrient solution improved by Yoshida et al. 1976 is (μM): H 3 BO 3 19, Ca(NO 3 ) 2 4H 2 O 1000, NH 4 NO 3 429, (NH 4 ) 6 Mo7O ...

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Abstract

The invention discloses a method for the in-situ dynamic observation and the measurement of the root system of a plant, which comprises the following steps: designing a transparent cultivation container and a growth medium; sterilizing seeds, accelerating germination, moving seedlings to the transparent cultivation container after budding, and keeping the natural growth of the root system of the plant in the transparent growth medium; scanning the root system by a three-dimensional laser scanning instrument, carrying out the in-situ observation and the image acquisition of the three-dimensional configuration of the root system and completing the steps of image collection, reconstruction, simulation and the like. By the three-dimensional visualization analysis of the in-situ growth condition of the root system of the plant, the invention directly reflects the three-dimensional growth condition of the root system, does not need destructive detection and realizes dynamic observation.

Description

technical field [0001] The invention belongs to the fields of root biology, plant nutrition, crop cultivation and plant physiology, and specifically relates to an in-situ dynamic observation and measurement method for plant roots. Background technique [0002] The root system is the main organ for plants to absorb nutrients and water, and plant root architecture refers to the spatial distribution of roots in the growth medium (Lynch, 1995). Different root architecture can significantly affect the absorption and utilization of nutrients and water (Fitter, 1987; Bowman et al, 1998; Doussan et al, 1998; Liao et al, 2001; Lambers et al, 2006; Skaggs and Shouse, 2008), carbon source Important physiological functions such as distribution (Nielsen et al, 1994; Bidel et al, 2000; Coll et al, 2008) and the ability to adapt to environmental stress (Miller, 1986; Al-Ghazi et al, 2003). Therefore, it is of great significance to study the three-dimensional structure of plant roots for p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01G31/00A01G31/02C05G1/00
CPCY02P60/21
Inventor 方素琴廖红严小龙
Owner SOUTH CHINA AGRI UNIV
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