Anabaena PCC7120 fat oxygenase gene

Abstract

The invention relates to an anabaena PCC7120 fat oxygenase gene which belongs to the biologic technical field of the food industry. The gene is derived from anabaena sp. PCC7120 and is multiplied andobtained from genome DNA by PCR, colibacillus is converted after an expression vector is inserted and is induced to express, and the expressed recombinant enzyme catalyzes the oxidation of unsaturatedfatty acid to generate a fatty acid hydrogen overoxidation derivative with conjugated double bonds. The hydroperoxide is used as an unstable primary enzyme promoting product and can further generatea series of oxidation secondary reactions. In the improvement of the quality of flour, the hydroperoxide has an important application value in the following two aspects: (1) oxidizing cysteine with reducibility, forming a disulfide bond, applying to the improvement of the quality of the flour, enhancing the strength of gluten and replacing potassium bromate with a carcinogenic action; and (2) oxidizing pigments, applying to the bleaching of the flour and replacing benzoyl peroxide.

Description

1. Technical Field [0001] The invention relates to an Anabaena PCC7120 lipoxygenase gene, which belongs to the field of food industry biotechnology and is dedicated to the cloning and high-efficiency expression of the Anabaena lipoxygenase gene. 2. Background technology [0002] Potassium bromate, as a quality improver for bakery industrial bread and cake flour, has been successfully applied abroad, especially in Europe and America, for decades. As a slow oxidant, it improves dough structure and rheology, strengthens gluten and elasticity, and obtains satisfactory results for baked products. my country has also earlier adopted potassium bromate as a flour quality improver and listed it in the GB2760 hygienic standard for use with a limit of 0.03g / kg. But it is stipulated that there must be no residue after baking. In the early days, it was believed that potassium bromate would be decomposed after baking, but in the 1980s in Japan and the United Kingdom, long-term studies have f...

AI Technical Summary

Problems solved by technology

Recombinant LOX has not been applied so far, and its biggest technical bottleneck is that the activity of LOX obtained from the fermentation of genetically engineered bacteria is extremely low, and most of them appear in the form of inactive inclusion bodies, which require renaturation, which greatly increases the cost.

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