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Method for producing hyaluronic acid (HA) by streptococcus zooepidemicus fermentation

A technology of Streptococcus zooepidemicus and hyaluronic acid, applied in the field of microbial fermentation to produce hyaluronic acid, can solve the problems of increasing energy consumption, increasing production costs, and being unfavorable to HA

Inactive Publication Date: 2010-01-06
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of fermenting and producing HA, as the fermentation proceeds, the viscosity of the fermented liquid becomes larger and the dissolved oxygen is limited, which inhibits the formation of HA
Although increasing the stirring speed can increase dissolved oxygen, it also increases energy consumption, increases production costs, and is not conducive to the formation of HA

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Streptococcus zooepidemicus (Streptococcus zooepidemicus) HA-116 ATCC 39920 is used as the starting strain to produce hyaluronic acid through liquid fermentation. The fermentation medium consists of: glucose 2% (mass percentage), peptone 1% (mass percentage) , yeast extract 0.5% (mass percentage), KH 2 PO 4 0.2% (mass percentage), MgSO 4 ·7H 2 O 0.05% (mass percentage), pH 7.5, sterilized at 121°C for 30min. The technological conditions are as follows: the inoculation amount is 10% (volume percentage content), and the cultivation temperature is 37.5°C. The addition amount of n-hexane is: 0.1% (volume percentage in the fermentation broth), and the addition time of hyaluronic acid is when the fermentation starts. After 48 hours of fermentation, the content of hyaluronic acid in the obtained culture solution was 0.352 g / L.

Embodiment 2

[0015] Streptococcus zooepidemicus (Streptococcus zooepidemicus) HA-116 ATCC 39920 is used as the starting strain to produce hyaluronic acid through liquid fermentation. The fermentation medium consists of: glucose 2% (mass percentage), peptone 1% (mass percentage) , yeast extract 0.5% (mass percentage), KH 2 PO 4 0.2% (mass percentage), MgSO 4 ·7H 2 O 0.05% (mass percentage), pH 7.5, sterilized at 121°C for 30min. The technological conditions are as follows: the inoculation amount is 10% (volume percentage content), and the cultivation temperature is 37.5°C. The addition amount of n-hexane is: 0.5% (volume percentage), and the addition time of hyaluronic acid is when the fermentation starts. After 48 hours of fermentation, the content of hyaluronic acid in the obtained culture solution was 0.374g / L.

Embodiment 3

[0017] Streptococcus zooepidemicus (Streptococcus zooepidemicus) HA-116 ATCC 39920 is used as the starting strain to produce hyaluronic acid through liquid fermentation. The fermentation medium consists of: glucose 2% (mass percentage), peptone 1% (mass percentage) , yeast extract 0.5% (mass percentage), KH 2 PO 4 0.2% (mass percentage), MgSO 4 ·7H 2 O 0.05% (mass percentage), pH 7.5, sterilized at 121°C for 30min. The technological conditions are as follows: the inoculation amount is 10% (volume percentage content), and the cultivation temperature is 37.5°C. The addition amount of n-hexane is: 0.5% (volume percentage in the fermentation broth), and the addition time of hyaluronic acid is when the fermentation proceeds to 12 hours. After 48 hours of fermentation, the content of hyaluronic acid in the obtained culture solution was 0.406 g / L.

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Abstract

The invention discloses a method for producing hyaluronic acid (HA) by streptococcus zooepidemicus fermentation. In the method, normal hexane is added in the fermenting process of streptococcus zooepidemicus so that the content of the HA in a fermenting product is greatly improved. When the normal hexane accounting for 0.50 percent of total volume of fermentation liquor is added, the HA yield reaches a maximal value of 0.406 g / L and is improved by 33.56 percent compared with a comparison.

Description

Technical field [0001] The present invention relates to a method for producing hyaluronic acid, in particular to a method for producing hyaluronic acid through microbial fermentation. Background technique [0002] Hyaluronic Acid (HA), also known as hyaluronic acid, is an acidic macromolecule mucopolysaccharide. At present, hyaluronic acid is mainly obtained by microbial fermentation. The Chinese patent with publication number CN 86100913 discloses a hyaluronic acid-producing Streptococcus zooepidemicus, whose deposit number is HA-116 ATCC 39920. This strain has better ability to produce hyaluronic acid. However, in the process of fermentation to produce HA, as the fermentation proceeds, the viscosity of the fermentation liquid becomes larger and the dissolved oxygen is limited, inhibiting the formation of HA. Although increasing the stirring speed can increase dissolved oxygen, it also increases energy consumption, increases production costs, and is not conducive to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/46
Inventor 邓静吴华昌石岩昌
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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