Preparation method of polyporus polysaccharide
Polyporus polysaccharide and Polyporus polysaccharide technology are applied in the field of preparation of Polyporus polysaccharide, can solve the problems of serious pollution, low polysaccharide content, unfavorable large-scale production operation, etc., and achieve the effects of less pollution, simple operation and favorable large-scale production operation.
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Embodiment 1
[0015] Take 10Kg of Polyporus coarse powder, add 4 times the amount of water to decoct once, 2 hours each time, filter, combine the filtrate, concentrate, add ethanol to sink to alcohol content of 50%, let it stand for 24 hours, dissolve the precipitate with water, Filtrate, concentrate the filtrate, add it to the H-1020 macroporous adsorption resin column, elute with water, collect the eluate with 2 times the column volume, concentrate, dry at low temperature, and pulverize to obtain 561g of Polyporus polysaccharide, which is detected by spectrophotometry , with a purity of 95.6%.
Embodiment 2
[0017] Take 10Kg of the coarse powder of Polyporus sinensis, add 10 times the amount of water to decoct 3 times, 2 hours each time, filter, combine the filtrate, concentrate, add ethanol to sink to alcohol content of 70%, let it stand for 24 hours, and dissolve the precipitate with water. Filtrate, concentrate the filtrate, add it to an X-5 macroporous adsorption resin column, elute with water, collect 5 times the column volume eluate, concentrate, dry at low temperature, and pulverize to obtain 998 g of Polyporus polysaccharide, which is detected by spectrophotometry , with a purity of 92.1%.
Embodiment 3
[0019] Take 10Kg of Polyporus sativa powder, add 8 times the amount of water to decoct twice, 2 hours each time, filter, combine the filtrate, concentrate, add ethanol to sink to alcohol content of 60%, let it stand for 24 hours, and dissolve the precipitate with water. Filtrate, concentrate the filtrate, add to the AB-8 macroporous adsorption resin column, elute with water, collect 4 times the column volume eluate, concentrate, dry at low temperature, and pulverize to obtain 861g of Polyporus polysaccharide, which is detected by spectrophotometry , with a purity of 98.4%.
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