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Soluble fodder enzyme and production process thereof

A manufacturing method and soluble technology, applied in applications, animal feed, animal feed, etc., can solve the problems of enzyme activity inactivation, etc., achieve the effect of small enzyme activity loss, reduce the loss of enzyme activity, and avoid the loss of enzyme activity

Active Publication Date: 2009-12-16
GUANGDONG VTR BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Secondly, this type of liquid product generally contains more than 80% water, and high temperature above 40°C often occurs during storage and transportation, and the enzyme activity of the product will be rapidly inactivated

Method used

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  • Soluble fodder enzyme and production process thereof
  • Soluble fodder enzyme and production process thereof
  • Soluble fodder enzyme and production process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Weigh 4.5kg of ZnSo4 7H2o, 1.5kg of anhydrous CaCl2, 40kg of sorbitol, 40kg of lactose, 20kg of β-cyclodextrin and 3kg of sodium carboxymethylcellulose, and slowly add it to 440Kg of phytic acid with an enzyme activity of 20676u / g In the enzyme concentrated solution, heat while stirring, and control the temperature to be less than 40°C. After all the solids are dissolved, the liquid is spray-dried to obtain 115Kg of phytase soluble powder with an enzyme activity of 55000u / g. This product is easily soluble at 5-10°C while stirring, and the solution is clear and transparent after dissolution.

[0040] Figure 13 Shown is the stability analysis of the phytase in this product stored at room temperature for 6 months. According to the analysis and calculation, the enzyme activity retention rate of the phytase in this product stored at room temperature for 6 months is 98%. Figure 14 Shown is the stability analysis of the phytase in this product stored at a constant temperatu...

Embodiment 2

[0042] Weigh ZnSo 4 ·7H 2 o 3kg, anhydrous CaCl 2 1kg, 30kg of sorbitol, 30kg of lactose, 10kg of β-cyclodextrin and 1kg of sodium carboxymethyl cellulose, slowly add the above-mentioned substances to the concentrated solution of 400Kg (xylanase concentrated solution containing 55000u / g enzyme activity 200kg and 13330u / g cellulase concentrate), heated while stirring, and controlled the temperature to be less than 40°C. After all the solids were dissolved, the liquid was spray-dried to obtain xylan with an enzyme activity of 110000u / g 80Kg of soluble powder of cellulase enzyme and enzyme activity 25000u / g. This product is easily soluble at 5-10°C while stirring, and the solution is clear and transparent after dissolution.

[0043] Figure 15 Shown is the stability analysis of the xylanase in this product stored at room temperature for 6 months. The analysis and calculation shows that the enzyme activity retention rate of xylanase in this product is 96% when stored at room ...

Embodiment 3

[0045] Weigh ZnSo 4 ·7H 2 o 6kg, anhydrous CaCl 2 3kg, 50kg of sorbitol, 50kg of lactose, 30kg of β-cyclodextrin and 5kg of sodium carboxymethyl cellulose, and slowly add the above-mentioned substances to the concentrated solution of 500Kg (the concentrated solution of xylanase containing 107276u / g enzyme activity 200kg, 200kg of cellulase concentrate with enzyme activity 27363u / g and 100kg of β-glucanase with enzyme activity 31606u / g), heat while stirring, and control the temperature to be less than 40°C. After all the solids are dissolved, it must contain 150Kg of soluble powder of xylanase with an enzyme activity of 115000u / g, cellulase with an enzyme activity of 27500u / g and β-glucanase with an enzyme activity of 18000u / g. This product is easily soluble at 5-10°C while stirring, and the solution is clear and transparent after dissolution.

[0046] Figure 19 Shown is the stability analysis of the xylanase in this product stored at room temperature for 6 months. The an...

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Abstract

The invention discloses a soluble fodder enzyme capable of effectively inhibiting bacterial reproduction, and lowering loss of the activity of the enzyme. The invention is a soluble powder prepared by adding soluble carriers into one or more condensed enzyme liquors containing one or more kinds of enzymes, spray drying after dissolving. The weight ratio between the condensed enzyme liquor and the soluble fodder enzyme is 400-500Kg of condensed enzyme liquor, 75-155Kg soluble carrier. The soluble carrier includes 3-6kg of zinc sulfate heptahydrate, 1-3kg of anhydrous calcium chloride, 30-50kg of sorbitol, 30-50kg of lactose, 10-30kg of beta- cyclodextrin, and 1-5kg of sodium carboxymethyl cellulose. The manufacturing method of the invention is that the soluble fodder enzyme powder is prepared by adding the soluble carrier slowly into the condensed enzyme liquor, stirring while heating and controlling the temperature to be not more than 40 DEG C., spraying and drying the liquor. The invention can be applied to the field of fodder additive.

Description

technical field [0001] The invention relates to a feed additive, in particular to a soluble feed enzyme and a manufacturing method thereof. Background technique [0002] Enzymes are biocatalysts with high specificity, about 100-1000 times that of chemical catalysts under comparable conditions. The enzyme (E) and the substrate (S) form an enzyme-substrate complex (complex) (E+S→ES) and then undergo a chemical reaction to generate a product (P) that is decomposed from the active site of the enzyme (ES→P+E ). Feed enzyme preparation, as a grain-saving and environment-friendly feed additive product, can play a unique role in solving problems such as animal husbandry and feed industry food shortage and environmental pollution. However, temperature and humidity have a great influence on the enzyme activity of feed enzymes. Generally, enzymes begin to inactivate when the humidity is 12% and the temperature is 60 degrees. In the process of feed processing, especially pellet feed,...

Claims

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Application Information

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IPC IPC(8): A23K1/165A23K20/189
Inventor 肖渲雄
Owner GUANGDONG VTR BIO TECH
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