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Method for extracting cholesterol from raw egg yolk

A cholesterol and egg yolk technology, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of nutritional balance imbalance, anemia and other diseases, prevent egg denaturation, facilitate drainage separation, and simple operation Effect

Inactive Publication Date: 2009-12-09
张志祥
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Because many foods containing cholesterol are also rich in other nutrients, if you avoid such foods too much, it is easy to cause nutritional imbalance, leading to anemia and other diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] 1. Process flow:

[0021] 1. Take 100kg of eggs and wash them, separate the egg white and egg yolk, and separate out 25kg of egg yolk, 62.5kg of egg white and 12.5kg of eggshell.

[0022] 2. Mix 25kg of fresh egg yolk and 125-250g of salt evenly in the container.

[0023] 3. Slowly add 50kg of absolute ethanol at 55-65°C under stirring conditions, seal it, and let it stand at an ambient temperature of 15-30°C for 6-8 hours, then use a filter cloth to filter out the filtrate, that is, the ethanol solution. Then centrifuged to obtain solid phase A and liquid phase A respectively.

[0024] 4. Uniformly mix the mixed solvent of n-hexane and ethanol with a volume ratio of 70:30 at 55-65°C and the pulverized solid phase A (the ratio of the mixed solvent to the solid phase A is 2:1), seal it, and place it at 15-30 After standing at ambient temperature for 2-3 hours, filter, remove the filtrate, centrifuge again, and keep the liquid phase B.

[0025] 5. Combine the two liqui...

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Abstract

A method for extracting cholesterol from raw egg yolk relates to the technical field of biological engineering. Fresh egg yolk, table salt and absolute alcohol are mixed, sealed and stood, filtering is carried out to remove the filtrate, and centrifugal separation is carried out to respectively extract solid phase and liquid phase; n-hexane, alcohol and solid phase are mixed, sealed and stood, then filtering is carried out to remove the filtrate, and centrifugal separation is carried out to extract the liquid phase; the two liquid phases are combined and stood for layering to extract the upper layer; absolute alcohol and the upper layer substance are evenly mixed and precipitated for 1-2 hours, then the supernatant is removed; under the environmental condition of 25-30 DEG C, filter cloth of 400-500 mesh is used for filtering out yolk oil, the residual mixture is placed in a vessel for heating up to 50-55 DEG C, then filter cloth of 400-500 mesh is used for filtering out phosphatidylcholine, the remainder is cholesterol. The method of the invention is easy to operate, and eggs per 100kg can prepare 550-600g of cholesterol by tests, featuring high yield, easy preservation and no peculiar smell.

Description

technical field [0001] The invention relates to the technical field of modern bioengineering extraction and processing. Background technique [0002] Cholesterol, also known as cholesterol, is widely present in animals, especially in the brain, nervous system and endocrine system cells and tissues, and is also high in kidney, lung, liver and bile. Cholesterol is an indispensable and important substance in animal tissue cells. It not only participates in the formation of cell membranes, but also is a raw material for the synthesis of bile acids, vitamin D and steroid hormones. [0003] There are two distinct and one-sided views on the role of dietary cholesterol. One point of view is that cholesterol is not harmful to the human body, and people can take it as much as they want. The reason why this view is wrong is due to the lack of understanding of the pathogenesis of hyperlipidemia and coronary heart disease. Long-term excessive dietary cholesterol intake will lead to th...

Claims

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Application Information

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IPC IPC(8): C07J9/00C07H1/08A23L1/29A23L33/00
Inventor 张志祥
Owner 张志祥
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