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Application of palmatine in medicine for treating West Nile virus infection

A technology of West Nile virus and palmatine, applied in the field of biomedicine, can solve the problems that cannot be completely ruled out, and there is no in-depth research, so as to achieve the effect of easy access and clear goals

Inactive Publication Date: 2009-12-09
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No clinical cases of WNV infection have been found in China so far, but this does not make us relax our vigilance against the virus, because China's climate and geographical environment are complex, and there are many kinds of mosquitoes, which have the conditions for transmission. At the same time, with the increasing frequency of international exchanges, WNV It is very likely that it will be introduced into China
In addition, there are a considerable number of cases of viral encephalitis of unknown etiology in China every year, because there is no in-depth research on WNV in China
Therefore, the possibility of existing WNV infection cases cannot be completely ruled out, and we should be vigilant against WNV

Method used

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  • Application of palmatine in medicine for treating West Nile virus infection
  • Application of palmatine in medicine for treating West Nile virus infection
  • Application of palmatine in medicine for treating West Nile virus infection

Examples

Experimental program
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Effect test

Embodiment 1

[0055] A kind of palmatine is used as the application in preparation treatment or prevention West Nile virus infection medicine, and its steps are:

[0056] B. Expression and purification of NS3 protein:

[0057] The plasmid pET28a-WNV-NS2BCF44NS3 (encoded with NS3 gene, donated by Dr. Shi Peiyong of Wadsworthcenter, USA, the gene and material must indicate the source of the document, and the gift cannot be written) was transferred into E.coli BL21 (DE3) (AMERSHAM, USA) with Inoculate the inoculation loop into solid LB medium containing 30 μg / ml Kan (AMRESCO, USA). After a single colony grows, pick a single colony and inoculate it in liquid LB medium containing 30 μg / ml Kan. Overnight at ℃ (12-16h), inoculate 1% (v / v) of the bacterial solution overnight at 2-8℃ in 1L of fresh LB liquid medium containing 30μg / ml Kan on the next day and expand the culture to OD 600 When the concentration is 0.60, add IPTG (Sigma, USA) with a final concentration of 0.5mmol / L, and incubate at 16°...

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Abstract

The invention discloses an application of palmatine for preparing medicine for treating and preventing West Nile virus infection; the palmatine to West Nile virus (WNV) has good inhibiting function, and the inhibition ratio of palmatine to the West Nile virus (WNV) is 97.71 percent respectively under the condition that the concentration of the palmatine is 100M, and the survival rate of Vero cells is 92.93 percent under the condition that the concentration of the palmatine is 100M. The palmatine can effectively inhibit West Nile virus, but has no cytotoxicity, therefore, the palmatine can be further developed into the medicine for treating diseases caused by West Nile virus infection and has wide application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to the application of palmatine in the preparation of medicines for treating or preventing West Nile virus infection. Background technique [0002] West Nile Virus (WNV) was first discovered in Uganda, Africa in 1937. It was isolated from the blood of a febrile adult woman in the West Nile area, hence the name West Nile Virus. It is widely distributed in Africa, Europe, Australia, Asia and the United States, Canada, Mexico, Caribbean and Central America and South America in the Western Hemisphere, mainly causing West Nile fever. Birds are the storage host of the virus and the main source of infection of the virus, and mosquitoes are the vector of the virus. At present, the virus has been detected in many birds. After the mosquito bites the infected bird, the virus exists in the salivary gland of the mosquito after 10-14 days in the mosquito body, and the virus can be tran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4375A61P31/12
CPCY02A50/30
Inventor 袁志明贾凡邹罡李晶石沛勇郑大胜蔡全信闫建平
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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