Method for detecting organophosphorus and carbamate pesticide residue

Abstract

The invention discloses a method for detecting organophosphorus and carbamate pesticide residue, which is to determine whether a sample to be tested contains organophosphorus or / and carbamate pesticides according to the suppression ratio of an extracting solution of the sample to be tested on cholinesterase, wherein the suppression ratio of the extracting solution of the sample to be tested on the cholinesterase is determined through the chromatic value of the extracting solution of the sample to be tested. The chromatic value is acquired through digital image processing technology. The method greatly improves detection efficiency, is particularly suitable for the mass detection of vegetable samples, and has the advantages of high automation degree, high measurement accuracy, quick, simple and convenient operation, few needed instruments and equipment, low cost, low requirement on environmental conditions of the determination, and no pollution.

Description

technical field [0001] The invention relates to a method for detecting residues of organophosphorus and carbamate pesticides. Background technique [0002] At present, the detection methods for organophosphorus and carbamate pesticides mainly include thin-layer chromatography, gas chromatography, and spectrophotometry. The first two methods have high detection accuracy and can achieve quantitative detection of each pesticide. , the instrument is expensive and requires special personnel to operate, so it is difficult to meet the market demand of fast, simple and easy operation. The national standard GB / T 5009.199-2003 stipulates that the detection of organophosphorus and carbamate pesticides in food adopts the enzyme inhibition rate method. The method is to determine whether the test sample contains organophosphorus or carbamate pesticides according to the inhibitory rate of the test sample extract to the cholinesterase. The principle of this method is: under normal circums...

AI Technical Summary

Problems solved by technology

[0003] Although the enzyme inhibition rate method used in the national standard has high sensitivity and low detection limit, and can quickly judge whether the residues of organophosphorus and carbamate pesticides in vegetables, fruits and other agricultural products exceed the standard, the specified method It is necessary to measure the absorbance value before the reaction and after three minutes of reaction, which has high requirements for the operation of the spectrophotometer, otherwise the color development time of the blank sample and the test sample will be deviated due to the time delay, thus introducing errors

This makes this method have certain limitations in practical application, and it is impossible to accurately determine the pesticide residues.

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